Organizing co mmittee

 
 

---

Poster presentation 
179 
SEC LIQUID CHROMATOGRAPHY OF CISTANCHE Salsa 
POLYSACCHARIDES 
 
Sh.Sh.
 
Orazova
1
, G.E. Berdimbetova
1
, A.S. Boymirzaev
2
, T.R. Egamberdiev
1
,
K.S.
 
Jaunbaeva
3
 
 
1) Karakalpak Research Institute of Natural Sciences of the Karakalpak Branch of 
the Academy of Sciences of the Republic of Uzbekistan, Nukus 
2) Namangan Institute of Engineering and Technology, Uzbekistan 
Nukus, st. Berdak №41, 
e-mail: [e-mail],
 tel. [phone] 
3) S. Yu. Yunusov Institute of the Chemistry of Plant Substances, 
Acaderny of Sciences of the Republic of Uzbekistan, 
e-mail: [e-mail]
 
In recent years, 
Cistanche salsa
(C.A. May) Beck has been actively studied in the 
field of vision, medicine and pharmaceuticals, as it contains various biologically active 
substances, including polysaccharides, phenolic compounds and a large number of 
flavonoids. Polysaccharides differ in their structure, physicochemical properties, etc. 
Monosaccharide composition, type of glycosidic bond, molecular weight and solubility 
in water or alkali. Biological activity depends on molecular parameters and structure. 
An analysis of the literature showed that information on the polysaccharides of many 
representatives of the genus 
Cistanche salsa
(C.A. May) Beck is limited or absent, so it 
is necessary to study the molecular weight of the polysaccharide of this genus. 
The purpose of the study is to determine the physicochemical properties of 
polysaccharides in the aerial part of the cistanche. 
For the study, the aerial part of the 
Cistanche salsa
plant, collected in April 2022 in 
the Nazarkhan forestry of the Kipchak department of the Amudarya district of the 
Republic of Karakalpakstan, was taken. 
Size exclusion liquid chromatography (SLC) of the obtained polysaccharides WSP 
and PS was performed on an Agilent 1260 Infinity high-speed liquid chromatograph 
(USA) with a refractometric detector. TSK GM PW XL was used as a sorbent with a 
linear calibration dependence in the molecular weight separation range from 1102 to 
2106. The eluent flow rate was 0.8 ml/min. The volume of the injected sample is 25 µl. 
The chromatographic data were processed using the Windows Chemstation 7 program. 
A 0.1 M aqueous solution of sodium nitrate served as the eluent. Gel chromatograms of 
two samples consist of three fractions of polysaccharides in the aerial part of cistanche. 
1 - the peak on the chromatogram characterizes pectin with a molecular weight of 12 
kDa, the remaining fractions are oligosaccharides with 3 and 1 kDa, respectively.
Shown are gel chromatograms of two samples obtained from the aerial part of cistanche, 
consisting of three chromatographic peaks of polysaccharide fractions with molecular 
weights of 25, 3 and 1 kDa. pectin substances isolated from the underground part of 
cistanche have a higher molecular weight (25 kDa) than from the aboveground part. 
However, in the samples of polysaccharides isolated from the aboveground and 
underground parts of the cistanche, there are fractions of oligosaccharides with an MM 
of 3 kDa and 1 kDa. To calculate the average MW of the samples, the universal Benois 
calibration dependence for pectin substances and pullulan standards was used. The 
following Mark-Kuhn-Houwink equations for pulluans were used to construct the ICP: 
[η]=1.9 10-4 М0.67; for pectins [η]= 0.18 10-4 М0.99 in water. 

---

Poster presentation 
180