Organizing co mmittee

 
 

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Poster presentation 
187 
GROWTH AND MAINTENANCE STUDIES OF
VERO CELL LINES
 
 
N. Tosheva, Sh. Khasanov, J. Abdurakhmanov, O. Ashirov, S. Sasmakov,
E. Yusupova, Sh. Azimova 
 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail] 
 
Vero cells are derived from the kidney of the African green monkey and are one of 
the most frequently used continuous mammalian cell lines in microbiology and 
molecular and cell biology research. Vero cells have been licensed in the United States 
for production of both live (rotavirus, smallpox) and inactivated (poliovirus) viral 
vaccines, and throughout the world Vero cells have been used for the production of a 
number of other viruses, including Rabies virus, Reovirus and Japanese encephalitis 
virus. The protocols outlined in this appendix detail procedures for the routine growth 
and maintenance of Vero cells in a research laboratory setting. In our study, we 
investigated the growth conditions of Vero cell lines under laboratory conditions. 
For long-term storage, Vero cells are stored in liquid nitrogen or at -80°C. We 
investigated the optimal conditions for growing Vero cells obtained from frozen stocks. 
The vial (cryovial) of Vero cells was rapidly thawed by gently swirling in a 38°C 
water bath. We transferred the Vero cell suspension from a cryovial to a 15 ml conical 
tube containing 5 ml DMEM supplemented with FBS. Pelleted cells were centrifuged at 
300 × g for 4 min at room temperature. The supernatant was removed and 5 ml of 
DMEM supplemented with 10% FBS was added. After freezing, Vero cells are best 
recovered in a small (25 sm
2
or 50 sm
2
) tissue culture flask. We used 25 sm
2
flasks, 
resuspended the cells in 5 ml medium. The flasks were incubated in an incubator at 
37°C with 5% CO2. Cells are monitored daily. The media is changed every 2-4 days. 
When cells reach more than 90% confluent monolayer, cells are transferred to new 
tissue culture flasks.