Organizing co mmittee

 
Reference 
1.
D. Kumar, V. Arya, Z. Ali Bhat, N. Ahmad Khan, D. Nandan Prasad. The genus 
Crataegus: chemical and pharmacological perspectives. 
Revista Brasileira de 
Farmacognosia Brazilian Journal of Pharmacognosy. 
V. 22 (5), 1187-1200 (2012).
 
 

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Poster presentation 
176 
STRUCTURAL ELUCIDATION OF TRITERPENE GLYCOSIDE 
FROM THE ROOTS OF Allochrusa gypsophiloides 
 
R.Z. Mamadalieva, V.U. Khujaev
 
Kokand State Pedagogical Institute, Kokand 713000, Turon str. 23, Uzbekistan 
e-mail: [e-mail] 
 
Allochrusa 
is a genus in the 
Caryophyllaceae
family
, which previously was included 
in the genus 
Acanthophyllum
. 
Allochrusa gypsophiloides
(Regel) Schischk. is the best 
known endemic saponin-bearing plant of Central Asia. This species has been known as 
a valuable source of saponins in Uzbekistan and Kazakhstan and has been exported for a 
long time.
Allochrusa
are a rich source of triterpene glycosides with diverse chemical 
structures and physiological activities.
A. gypsophiloides
is represented natural resource of the metabolites, especially 
triterpene saponins and polysaccharides. Although, there are some reports about 
bisdesmoside glycosides (acanthophyllosides B, C, and D) in this plant, chemical 
investigations have not completed on 
A. gypsophiloides
yet. Therefore, the study of root 
material led to the isolation of one triterpene glycosides not described previously in this 
plant. The structural elucidation of this triterpenoid saponin was based on mass-, NMR 
spectroscopic techniques.
 
The roots of 
A. gypsophiloides
were grounded and powdered afterward. The saponins 
were extracted using ultrasonic bath with MeOH (80%) at room temperature. The 
extract was then filtered and evaporated. The dried methanol extract was subjected to 
Diaion HP20 column chromatography, and the extract was eluted by H
2
O/MeOH 
gradients with increasing polarity to 100% MeOH. Triterpene glycosides-containing 
subfractions was chromatographed and compound