Organizing co mmittee

ENDOPHYTES FROM LICORICE - Glycyrrhiza glabra AS 
BIOCONTROL AGENTS AGAINST Fusarium CAUSED DISEASES 
 
G.U. Mardonova, D.R. Egamberdieva 
National University of Uzbekistan, University street-4, 100174, Tashkent, Uzbekistan 
 
The objective of this research was to reveal bacterial endophytes isolated from 
Licorise-
Glycyrrhiza Glabra Linn
plant, which have biocontrol activity against 
phytopathogenic fungi 
Fusarium tricinctum
and 
Fusarium culmorum
.
The bacterial endophytes were isolated from shoots and roots of Licorise. The endophytes 
were isolated after grinding of the surface sterilized roots or shoots, dilution of the cell juice in 
phosphate buffered saline and spreading the suspension on Nutrien Agar (NA) and Yeast 
Extract Mannitol (YEM). The bacterial isolates were identified using 16S rRNA gene 
analysis. For DNA extraction the heat treatment method was used. Extracted DNA was used 
as template for 16S rRNA gene analysis. Sequencing was performed using ABI PRISM 
BigDye 3.1 Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems). The 
16S rRNA sequences were identified using BLAST and comparisons with the GenBank 
nucleotide data bank from the NCBI. The grown colonies were picked up in 4 days. The 
endophytes were checked for in vitro antagonistic activity against phytopathogenic fungi 
Fusarium tricinctum
and 
Fusarium culmorum
on Petri dishes using Chapek medium. The 
endophytes were examined for the ability to inhibit the same phytopathogenic fungi in pot 
experiment.
In total 29 isolates were obtained from Licorise plants. Only 10 isolates inhibited 
fungi 
Fusarium tricinctum
and 
Fusarium culmorum
or both of them. IYB2, PYB4, 
IPB10, INB18 bacterial isolates were the most effective in biocontrol activity against 
disease caused by 
Fusarium tricinctum
pot experiments. The most effective bacterial 
isolates having biocontrol activity against disease caused by 
Fusarium culmorum
were 
PYB1, PNB15 and INB9. In total 10 bacterial isolates were identified using 16S rRNA 
gene analysis as follows: 
Pantoea agglomerans
PYB1, Achromobacter sp. PNB21, 
Bacterium AM0120 
IYB3, 
Klebsiella oxytoca
IYB1, 
Enterobacter hormaechei
IYB2, 
Pantoea agglomerans
PYB4, 
Pantoea sp
IPB10, 
Pseudomonas azotoformans 
INB18, 
Enterobacter ludwigii
PPB13 and 
Bacillus siamensis 
INB9. 
№ Endophytes of Licorise 
Fungal growth inhibition zone (mm) 
Fusarium tricinctum
 
Fusarium culmorum
1 
PYB1 
- 
5 
2 
PNB21 
4 
- 
3 
IYB3 
3 
- 
4 
IYB1 
2 
3 
5 
IYB2 
5 
4 
6 
PYB4 
6 
- 
7 
IPB10 
7 
- 
8 
INB18 
5 
9 
PPB13 
- 
8 
10 
INB9 
- 
5 
The strains possessing high biocontrol activity towards phytopathogenic fungi can be 
proposed for usage as a part of biofungicides after field experiments. 

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Poster presentation 
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