v 5,
3, (2019)
Poster presentation
170
MULTIFUNCTIONAL POLYSACCHARIDE HYDROGELS FOR
WOUND HEALING
A.A. Boydedayev
1
, B.I. Muhitdinov
1,2
, D.M. Amonova
1
, A.S. Turaev
1
, Y.Huang
2
,
I.Y. Mexmonov
1
, B.A. Sindarov
1
, M.O. Kalonova
1,3
, L.U. Makhmudov
1
,
H. Wang
2
1) Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Mirzo Ulugbek
St. 83, 100125 Tashkent, Uzbekistan
2) Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Hai Ke
Road, 201203 Shanghai, China
3) National University of Uzbekistan, 4 University St. 4, 100174 Tashkent, Uzbekistan
The wound healing processes include complex biological and physiological
mechanisms such as hemostasis, inflammation, proliferation, and remodeling. Wound
healing is frequently considered a serious social problem in burned and diabetic patients
especially. However, there are very limited therapeutic materials in use and thus
effective wound-healing materials are still have been required. Polysaccharides usually
are considered suitable biopolymers for developing wound healing materials with their
unique biocompatibility, biodegradability, and high water uptake and retention
properties. Among others, hyaluronic acid is a structural and functional biopolymer,
largely presents in pericellular coatings and extracellular matrix, an important ingredient
of almost all tissue, and plays an important role in wound healing.
In this study, hyaluronic acid hydrogels containing the chitosan oligosaccharide and
β-glucan polysaccharide were developed. The hydrogel formulation was structured by
crosslinking the polysaccharide chains with chitosan oligosaccharides. The hydrogels
prepared were structurally analyzed with IR spectroscopy, differential scanning
calorimetry (DSC), and SEM observation methods. The wound-healing activities of the
samples were studied dorsal wound rat model.
In the IR spectra of the hydrogel samples changes in the peaks from amide (I/II/III)
were observed at 1310-1558 cm
-1
, confirming the crosslinked structure of the samples.
The DSC curves of the hydrogel samples differed from that of the initial polysaccharide
samples showing an endothermic absorption peak at 40-100°C, and exothermic
absorption peaks around 270-350°C due to differing chemical and physical composition
of the samples. The SEM observations of lyophilized hydrogels clearly demonstrated
that the hydrogels possess irregular macropores and wavy morphological structures. In
the wound permeability evaluations executed in the dorsal wound rat model, the β-
glucan containing hyaluronic acid-based hydrogel demonstrated a more progressive
wound healing effect (wound closure 100%) compared to the control (wound closure
52.3%) or the hydrogel without β-glucan (wound closure 86.7%) at day 14.
In conclusion, we through this study developed hyaluronic/chitosan/β-glucan
hydrogel for wound treatment applications. The composite hydrogel developed
demonstrated promising wound-healing activity in the animal model. The composite
hydrogel is expected to be multifunctional with respect to its applicability together with
other supporting or therapeutic payloads. The composite hydrogel is under study for
evaluation of detailed pharmacological properties.
Poster presentation
171
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