Organizing co mmittee

v 5,
3, (2019)
 
 

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Poster presentation 
170 
MULTIFUNCTIONAL POLYSACCHARIDE HYDROGELS FOR
WOUND HEALING 
 
A.A. Boydedayev
1
, B.I. Muhitdinov
1,2
, D.M. Amonova
1
, A.S. Turaev
1
, Y.Huang
2
, 
I.Y. Mexmonov
1
, B.A. Sindarov
1
, M.O. Kalonova
1,3
, L.U. Makhmudov
1
,
H. Wang
2
1) Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Mirzo Ulugbek 
St. 83, 100125 Tashkent, Uzbekistan 
2) Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Hai Ke 
Road, 201203 Shanghai, China 
3) National University of Uzbekistan, 4 University St. 4, 100174 Tashkent, Uzbekistan 
The wound healing processes include complex biological and physiological 
mechanisms such as hemostasis, inflammation, proliferation, and remodeling. Wound 
healing is frequently considered a serious social problem in burned and diabetic patients 
especially. However, there are very limited therapeutic materials in use and thus 
effective wound-healing materials are still have been required. Polysaccharides usually 
are considered suitable biopolymers for developing wound healing materials with their 
unique biocompatibility, biodegradability, and high water uptake and retention 
properties. Among others, hyaluronic acid is a structural and functional biopolymer, 
largely presents in pericellular coatings and extracellular matrix, an important ingredient 
of almost all tissue, and plays an important role in wound healing. 
In this study, hyaluronic acid hydrogels containing the chitosan oligosaccharide and 
β-glucan polysaccharide were developed. The hydrogel formulation was structured by 
crosslinking the polysaccharide chains with chitosan oligosaccharides. The hydrogels 
prepared were structurally analyzed with IR spectroscopy, differential scanning 
calorimetry (DSC), and SEM observation methods. The wound-healing activities of the 
samples were studied dorsal wound rat model.
In the IR spectra of the hydrogel samples changes in the peaks from amide (I/II/III) 
were observed at 1310-1558 cm
-1
, confirming the crosslinked structure of the samples. 
The DSC curves of the hydrogel samples differed from that of the initial polysaccharide 
samples showing an endothermic absorption peak at 40-100°C, and exothermic 
absorption peaks around 270-350°C due to differing chemical and physical composition 
of the samples. The SEM observations of lyophilized hydrogels clearly demonstrated 
that the hydrogels possess irregular macropores and wavy morphological structures. In 
the wound permeability evaluations executed in the dorsal wound rat model, the β-
glucan containing hyaluronic acid-based hydrogel demonstrated a more progressive 
wound healing effect (wound closure 100%) compared to the control (wound closure 
52.3%) or the hydrogel without β-glucan (wound closure 86.7%) at day 14.
In conclusion, we through this study developed hyaluronic/chitosan/β-glucan 
hydrogel for wound treatment applications. The composite hydrogel developed 
demonstrated promising wound-healing activity in the animal model. The composite 
hydrogel is expected to be multifunctional with respect to its applicability together with 
other supporting or therapeutic payloads. The composite hydrogel is under study for 
evaluation of detailed pharmacological properties.

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Poster presentation 
171