Organizing co mmittee

 
 

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Poster presentation 
198 
ISOLATION AND CHARACTERIZATION OF THERMOPHILIC 
BACTERIA FROM HOT SPRING IN OLTINSOY VILLAGE, 
NAVOIY REGION, UZBEKISTAN 
 
N.N. Shodiev
1
, S.A. Abdusamatov
1
, A.I. Kulonov
2
, K.V. Kondrasheva
2
,
K.D. Davranov
2 
 
1) National University of Uzbekistan named after Mirzo Ulugbek, 100174, Tashkent, 
Uzbekistan 
2) Institute of Microbiology, AS of Uzbekistan, 100128, Tashkent, Uzbekistan 
e-mail: [e-mail]  
 
Organisms that grow at high temperatures are known as thermophiles and typically 
associated with solar, geothermally, industrially or biologically heated environments. In 
general, they are divided into three categories according to their cardinal growth 
temperatures: thermophiles (35-70
0
C), extreme thermophiles (55-85
0
C), and 
hyperthermophiles (75-130
0
C). 
Enzymes found in thermophilic organisms are excellent sources of enzymes that can 
withstand high temperatures and carry out reactions efficiently. The discovery of 
extremophiles has been a remarkable impetus for biotechnology industries. The 
products secured from extremophiles such as proteins, enzymes (extremozymes) and 
compatible solutes have great biotechnological potential. The inherent stability of 
thermostable enzymes, which have been isolated mainly from thermophilic or 
thermotolerant organisms, has made them suitable candidates for a number of 
commercial applications. 
The main goal of our research is to find new strains of thermophilic bacteria and to 
determine their biotechnological potentials. It started with taking samples from hot 
springs in the village of Oltinsoy, Khatirchi district, Navoi region of Uzbekistan. In this 
case, water and sediment samples were taken from different parts of hot springs (mainly 
the beginning of the spring, 1-2 and 3-4 meters away from the basin), measuring 
temperature and pH values. The sterile samples were grown in M-162 nutrient medium 
and a total of 6 isolates were isolated. During the experiment, unknown cultures were 
incubated in M-162 nutrient medium in a thermostat at 60℃ for 2-5 days. Colony 
diameter, morphology and colony structure were recorded after 5 days of isolates. 
Colonies of bacterial isolates are characterized by the following characteristics: color, 
shape, height, border, diameter, surface, transparency and texture. In our next 
experiments, the isolates were stained by Gramm's method. It was observed that 3 out of 
6 isolates were gram positive and the remaining 3 were gram negative. Because of 
importance to determine the enzyme activity of thermophilic bacteria, enzyme activity 
such as Amylase, Protease, Gelatinase and Lipase was studied. Initially, our isolates 
were grown in liquid nutrient media for 2-3 days and inoculated on substrates grown in 
solid nutrient media. The results showed that strains 57W, 57S, 60W and 60S grown in 
specially selected M-162 medium showed high activity against Protease enzyme. None 
of the other strains showed activity against the remaining enzymes. 
In conclusion, it was shown that only 4 of the 6 isolated strains have a high activity 
against Protease enzyme, and this indicator is very low in the other strains. In our next 
researches, we plan to identify the isolates by studying them at the molecular level and 
to study more the biotechnological potentials of the strains with high activity. 

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Poster presentation 
199