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Oral presentation
31 
MOLECULAR GENETIC ANALYSIS OF THE EFFECT OF THE 
SUPRAMOLECULAR COMPLEX OF GLYCYRRHIZIC ACID 
WITH BENZOTRIOZOLE ON WHEAT FUNGAL DISEASES 
S.Kh. Hojiboboeva, M. Khudaynazarov, U.M. Shapulatov, Kh.Kh. Kushiev 
 
Gulistan State University, Uzbekistan 
We studied the effect of a supramolecular complex of glycyrrhizic acid (GA) with 
benzathriosol, toxic to Fusarium fungi, and elicitor metabolites that activate proton and 
salicylate-dependent signaling systems in plants and induce systemic resistance to 
diseases. The results of experiments in which the fungicidal treatment was preceded by 
the treatment of wheat seedlings with the complex indicated that these metabolites can 
also serve as sensitizers that enhance the fungicidal effect against 
Fusarium
diseases. 
In this case, we performed a molecular-genetic analysis of the level of fungal 
infection of wheat under the influence of supramolecular complexes of GK with 
benzotrazole (GK:BT) and aminotriazole (GK:AT), whose effective effect against this 
fungus was studied. Genomic DNA from wheat plants infected with fungi was extracted 
using the STAV method, and the DNA concentration was homogenized in a NanoDrop 
3300 (Thermo Fisher, USA) spectrophotometer. Genomic DNA samples extracted from 
wheat seedlings were analyzed by PCR. A DNA marker was used to compare the PCR 
product. The results showed that no fusarium DNA was detected in any of the control, 
non-fusarium wheat cultivars. Also, samples treated with control + GK:BT or GK:AT, 
but not infested with 
Fusarium
, were negative for 
Fusarium
DNA PCR reaction.
During our research, it was also clear that when wheat seeds were treated with 
GK:BT or GK:AT solutions and 
Fusarium mycelium
(F. Poae), the level of 
Fusarium
infection in the early stages of wheat seedlings was reduced. Patterns 1, 2, 3, and 4 in 
this study were observed in DNA samples extracted from October sprouts of winter 
wheat. In May, planted wheat samples enter the last stage of development, which has a 
great impact on productivity. It was at this stage that the PCR reaction to fusarium DNA 
showed a negative result in the plant samples isolated. 
The replicate number of expression in plants was analyzed by qRT-PCR method of 
similar samples. The extracted DNA samples were initially brought to the same 
concentration and subjected to a real-time reaction using the method recommended by 
OOO "AgroDiagnostika" (Russia). This reaction was carried out on the DT-96 amplifier 
(OOO "NPO DNA-technology") equipment. The results showed that the PCR product 
of a specific locus in the 
Fusarium
genome was observed to be lower in samples treated 
with GK:BT or GK:AT solutions. On the contrary, it was shown that the PCR product 
was higher only in wheat samples infected with fusarium.