Organizing co mmittee

1
-
3
and 
5
-
7
were found in 
this plant for the first time. A new C
20
-diterpene alkaloid named 15-epinaviculine B with 
a thalasamine skeleton has also been isolated from the aerial part of 
D. oreophilum
(
8
). 
1
. R
1
=Мe, R
2
=Me
5
6
7 
8
2
. R
1
=Н, R
2
=Ас
3
. R
1
=Mе, R
2
=Н
4
. R
1
=Mе, R
2
=Ас 
The structures of the isolated compounds were identified by 1D, 2D NMR 
spectroscopy, mass spectrometry, and the structure of alkaloids 
4, 5,
and 
8
was 
established by X-ray diffraction analysis. 
References:
1. Flora of Uzbekistan
. 
Vol. II, Tashkent, [in Russian], pp. 447–460 (1953). 
2. H.I. Ahunova, Sh.V. Abdullaev, M. Rajabova, 
Scientific bulletin of NamSU, 6, 
81 
(2019). 
3. V.G. Kazlikhin, V.A. Tel’nov, M.S. Yunusov, S.Yu. Yunusov, 
Chem. Nat. Compd
., 
13
, 737 (1977) 

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Oral presentation
27 
GENETIC ANALYSIS OF THE CRY GENES OF THE 
ENTOMOPATHOGENIC BACILLUS THURINGIENSIS AGAINST 
Helicoverpa armigera 
 
I.M. Khalilov, F.B. Kobilov, N.S. Akhmedova 
 
Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, 7b 
Abdulla Qadiri Street, Tashkent 100128 
Microorganisms are currently being used to produce ecologically green agricultural products. 
Among such microorganisms, 
Bacillus thuringiensis
(Bt) bacterium is the best biological agent 
against pest insects. For this reason, it is possible to achieve many desired results by 
comprehensively studying this bacterium's morphological and genetic characteristics. That is why 
this bacterium has been the focus of the attention of many scientists. 
Bacillus thuringiensis
is a gram-positive, rod-shaped, spore-forming bacterium found 
worldwide in soil, water, dead insects, hay, tree leaves, various conifers, and diverse 
ecosystems, including insectivorous mammals, as well as humans with severe necrosis. 
It has been found that it occurs and develops in the tissues and as a probiotic in the 
composition of cow's milk, in environments contaminated with phenolic compounds 
and various pesticides. An entomopathogenic feature of Bts is that they produce 
crystalline proteins (Cry). These proteins cause the death of harmful insects. Cry genes 
code these Cry proteins. 
Using the modified Marmur method, genomic DNA was isolated from 
Bt
strains, 
specific primers for cry1Aa-I, cry1Ab, cry1Ac-I, cry9U-I, and cry2 genes were 
designed and PCR amplification was performed. When the partial sequence of the 
cry1Ab gene of strain 
Bt94
was compared in the NCBI database, 93.53% similarity was 
observed with the genes numbered AY007686.1, M97880.1, and MT226609.1, and 
93.41% with the gene numbered EU679502.1. The cry1Ab gene was compared using 
Slustal Omega online software to find SNP variation.
According to the results of the analysis, it was found that the 
Bt94
cry1Ab gene was 
mutated at 32 points from the nucleotide sequence of other compared genes. In this 
case, at nucleotide 83 (T/C), at 87 (A/S), at 154 (T/S), at 195 (G/A), at 227 (T/G), at 282 ( 
G/A), 315 (G/S), 318 (T/S), 357 (A/S), 361 (A/S), 372 (S/T), 381 (A/G), 388 (G/A), 435 
(S/T), 440 (T/A), 461 (A/G), 493 (A /G), 501 (T/S), 517 (T/G), 552 (S/T), 555 (T/S), 564 
(S/T), 567 (T/G), 571 (G/A), 576 (A/G), 591 (G/A), 597 (S/A), 621 (T/ A), it was observed 
that there was a mutation at 674 (G/T), 682 (S/G) and 685 (A/G) nucleotides, and only 
the 
Bt94
cry1Ab gene changed from the others at these nucleotide positions with 20 
transitions and 12 transversions. the mutation was analyzed. 
It should be noted that the nucleotide sequence of the cry1Ab gene of the 
Bt94
strain, 
assuming that the primers encoding the cry1Ab gene also encode the cry1s gene, it can 
be seen in the NCBI database that the gene product identified as the cry1Ab gene 
belongs to the variant of the cry1s gene. 
Thus, when the partial genes 
Bt1
-cry1Ac, 
Bt18fo
-cry1Ab, 
Bt26
-cry1Aa, 
Bt31
-cry2, 
Bt84
-
cry1Ac, 
Bt91
-cry9U, 
Bt93
-cry9U, and 
Bt94
-cry1Ab were constructed, a phylogenetic tree 
was created using the Mega-4 statistical program. All identified genes were observed to 
match their corresponding cry gene classification. In addition, it was found that all studied 
genes are in 1 cluster, respectively cry genes. The identified partial cry genes are a good 
scientific resource for future whole-genome sequencing and expression studies. 

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Oral presentation 
28