Organizing co mmittee

5
):(
1
) – 
3:1, 10 mM potassium-phosphate buffer (pH 8.0), PNP - 1050 units, 50 ⁰С, the reaction 
time - 7 days. Yield of target compound 
2
- 
38%. 
This research was funded by the Russian Science Foundation (Project No. 21-13-
00429). 
N
N
N
N
NH
2
O
OH
OH
HO
F
N
N
N
N
NH
2
O
OH
HO
F
N
N
N
N
NH
2
O
OH
HO
H
2
N
+
PNP, 2-F-Ado
O
HO
OH
N
N
N
HN
O
Hyp
O
HO
OH
O
P
O
OLi
OLi
PNP, 2-F-Ado
method 1
method 2a
method 2b
2-F-Ado (1)
(2)
(3)
(4)
(5)

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Oral presentation
25 
STAUDYING THE POSSIBILITY OF OBTAINING PEKTIN FROM 
WASTE BY ENZYMATIC HIDROLYSIS 
 
B.A. Abylaeva, T.T. Zhumabaeva 
Osh State University, Kyrgyzstan, [e-mail] 
In medicine and veterinary medicine, the use of drugs, in particular antibiotics, has 
led to a decrease in the body's resistance to harmful factors and a change in the 
environment in which microorganisms live.
In this regard, the use of pectin as a prophylactic is very important. Therefore, it 
became necessary to obtain pectin from the waste of juice producers, wild varieties of 
apple trees, as well as the peel of the local pomegranate variety Kara-Kaiyn, which 
grows in the Aravan district of the Osh region, has not been studied. 
The purpose of the research is to study the possibility of obtaining pectin from plant 
waste by enzymatic hydrolysis. 
The release of pectin in large quantities depends on the complete hydrolysis of plant 
cell components. The use of cellulase leads to the hydrolysis of cellulose, which 
contributes to the separation of the complex of polygalacturonic acid and hemicellulose 
from the cell membrane and the hydrolysis of the latter increases the content of 
polygalacturonic acid in the isolated pectin. Pomegranate and mandarin peels were used 
as vegetable waste. When using these plant wastes as a substrate, there is an increase in 
the yield of pectin under the influence of the fungi 
Fomes fomentarius
and 
Pleurotus 
ostreatus
, which are 72.78% and 46.7%. The study of the maximum yield of pectin 
showed that when the conditions of enzymatic hydrolysis were changed, the 
temperature was 50°C, and the pH was 4.5. The enzyme is added in an amount of 
0.04% equivalent, in the ratio of the substrate used. Fruit pectins contain a sufficient 
amount of acetyl, carboxyl groups, which adversely affects the adhesion of pectin 
substances and creates a poor gel, while pomegranate, citrus, and apple pectins do not 
have such properties. Thus, research on the extraction of pectin once again showed that 
waste raw materials that are not used in practice and have a negative impact on the 
environment could be a promising raw material base for the production of surfactants 
using new technologies. Thus, research on the extraction of pectin once again showed 
that waste raw materials that are not used in practice and have a negative impact on the 
environment could be a promising raw material base for the production of surfactants 
using new technologies. It also allows the use of pectolytic enzymes to obtain an 
environmentally friendly product with a content of up to 40% compared to acid 
hydrolysis of fruit residues during pectin extraction. Therefore, the use of enzyme 
preparations of hemicellulase and cellulase allows increasing the yield of pectin and 
obtaining an environmentally friendly product, which in turn allows expanding the 
range of pectin applications.