Organizing co mmittee

References: 
1.
K. J. Livak, Genet Anal, 14 (5-6), 143 (1999) 
2.
Chloe James, Valerie Ugo, Jean-Pierre Le Couedic, Judith Staerk, Francois 
Delhommeau, Catherine Lacout, Loic Garcon, Hana Raslova, Stefan N. Constantinescu, 
Nicole Casadevall, William Vainchenker, Nature, 434 (7037), 1144 (2005) 

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Poster presentation 
317 
ON THE HYPOLIPIDEMIC EFFECT OF TOTAL EXTRACTIVE 
PREPARATIONS CONTAINING FLAVONOIDS, TRITERPENE 
GLYCOSIDES AND PHYTOECDYSTEROIDS ISOLATED FROM 
Thermopsis alterniflora, Zygophyllum oxianum and Silene viridiflora 
 
N.Kh. Yuldasheva
2
, D.A. Narbutaeva
1
, V.N. Syrov
1
 
1) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences 
of the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail] 
2) Tashkent Medecinal Academy, Farobiy 2, Uzbekistan, 
e-mail: [e-mail] 
 
Currently, the idea of using various biologically active substances isolated from 
plants for the prevention and treatment of hyperlipidemia and atherosclerosis is 
becoming more widespread. 
The aim of the present work was to study in an appropriate way the sum of 
flavonoids from 
Thermopsis alterniflora
, the sum of triterpene glycosides from 
Zygophyllum oxianum
and the sum of phytoecdysteroids from 
Silene viridiflora
. 
The experiments were carried out on male rats weighing 200-250 g, with 
hyperlipidemia caused by triton WR-1339 (225 mg/kg, once, intraperitoneally). The test 
substances were administered orally at doses of 5-50 mg/kg. The content of total 
cholesterol and triglycerides in the blood serum of animals was determined by the 
enzymocalorimetric method using Cypress diagnostics (Belgium) reagent kits on a 
Secomam Basic biochemical analyzer (France). In the course of the studies, it was 
found that the amount of flavonoids isolated from 
Thermopsis alterniflora
(apigenin, 
luteolin, formononetin, etc.), from 
Silene viridiflora
, the amount of phytoecdysteroids 
(ecdysterone, sileneosides A, D, polypodin B, etc.) and from 
Zygophyllum oxianum
, the 
amount of triterpene glycosides (zygofiloside E, etc.) have a significant hypolipidemic 
effect. The amount of triterpene glycosides from 
Z. oxianum
lowered the level of 
cholesterol in the blood serum more pronouncedly (by 35.0%), the amount of 
flavonoids from 
Th. alterniflora
clearly showed a hypotriglyceridemic effect of 40.8%, 
and the amount of phytoecdysteroids from 
S. viridiflora
had an approximately 
equivalent effect in this regard (25.8-30.2%). The reference drug clofibrate under these 
conditions lowered the level of cholesterol in the blood serum by 27.2%, and 
triglycerides by 38.6%. The data obtained in experiments on rats on a significant lipid-
lowering activity of the studied substances were subsequently confirmed in rabbits with 
experimental atherosclerosis caused by prolonged oral administration of cholesterol. 
Thus, the considered total preparations of flavonoids, triterpene glycosides, and 
phytoecdysteroids are of interest as potential drugs for the treatment of hyperlipidemia 
and atherosclerosis.