Organizing co mmittee

INFLUENCE OF THE COMBINATION OF CYTISINE WITH 
SUCCINIC ACID ON THE ACUTE ALCOHOLIC INTOXICATION 
 
Sh.B. Latypova
1
, A.A.
 
Azamatov
2
, F.M. Tursunkhodzhaeva
2
, Kh. Davronova 
 
1) Urgench branch of Tashkent Medical Academy, MH RUz 
2) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
 
S
uccinic acid-containing remedies belong to drugs of a metabolic type of action, the 
pharmacotherapeutic effects of which are aimed at restoring biochemical metabolic 
reactions disturbed by pathological processes. This type of drugs is widely used in 
cardiology, neurology and hepatology. The antihypoxic effect of SA is based not only 
on the ability to activate the succinate dehydrogenase pathway of ATP re-synthesis in 
the ischemic zone, to reduce the level of NAD-dependent substrates of the Krebs cycle 
and fatty acids, but is also associated with the stimulation of the cytochrome oxidase 
activity, which is a key enzyme of the respiratory chain of mitochondrial cells. 
Succinate-containing drugs, for example, Reamberin, in case of poisoning with 
neurotoxic poisons, reduce the time spent in a coma and patients’ death. 
Alcoholic coma is often accompanied by respiratory failure. We have previously 
established that cytisine and its derivative N-(3,4-methylenedioxybenzyl)cytisine 
hydrochloride have a pronounced antitoxic effect in acute alcohol intoxication, and N-
(3,4-methylenedioxybenzyl) cytisine hydrochloride is 2.8 times less toxic than cytisine 
(1). 
The purpose of this work is to study the effect of a combination of cytisine and succinic 
acid in a ratio of 1:1 on acute alcohol poisoning. Substances were injected subcutaneously for 
10-15 minutes before intraperitoneal injection of 24% ethanol at a dose of 4.8 g/kg. The effect 
of the substances was judged by the duration of the sleep of animals. The results are presented 
in the table, which shows that the studied composition has a pronounced antitoxic effect, 
exceeding the highest effects of cytisine and SA by 12 and 86%, respectively. 
Table. Effect of cytisine and its combination with UC on acute alcohol intoxication in mice 
(N=10) 
No 
Animal group 
Dose, 
mg/kg 
Sleep duration 
Effectiveness,% 
min. 
% 
1 
Control (ethanol)
4,8 
107.6±4.2 
100% 
- 
2 
Succinic acid (SA) 
5.0 
72.4±2.8 
67.2 
32.8 
10.0 
64.5±3.1 
59.9 
40.1 
3 
Cytisine (C) 
0.1 
36.1±2.4 
33.5 
66.5 
0.5