Organizing co mmittee

part of the nettle was 5.35 mg %. 
 
 

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Poster presentation 
149 
STUDIES OF TANNIES IN THE LIQUID EXTRACT 
“EXTRADENT” 
 
Sh.S. Yuldasheva, N.A. Yunuskhodjayeva, N.E. Yunuskhodjiyeva, 
D.R. Gulyamova
 
 
Tashkent Pharmaceutical Institute, st. Aybek 45, Tashkent, Uzbekistan
 
Ensuring the proper quality of medicinal products largely depends on the proper 
organization of control, its effectiveness and efficiency, as well as on the level of 
requirements laid down in the regulatory documentation (RD) and the methods of 
analysis used. We have developed an anti-inflammatory, hemostatic liquid extract from 
herbs Knotweed (
Polygonum hydropiper 
L.)
, 
shepherd's purse (
Bursa pastoris
)
, 
marigold flowers (
Calendula officinalis 
L
.
) and nettle leaves (
Urtica dioica 
L.). These 
plants contain various biologically active substances. The purpose of our study was to 
study the content of tannins in the liquid extract "Extradent". 
According to literary sources, we know that when determining tannins, the titrometry 
method is most widely used. We have developed an HPLC method for the identification 
and quantification of tannins using gallic acid. 
Gallic acid was determined using a SHIMADZU LC-2030 Plus high-performance 
liquid chromatograph. Separation is carried out on a 150

4.6 mm HPLC ACE 3 C18 
column with a particle size of 3 µm. Mobile phase water: acetonitrile at a ratio of 80:20, 
detection wavelength 272 nm, analysis time 30 minutes, pH of the mobile phase - 2.7, 
flow rate of the mobile phase - 1.0 ml/min, volume of injected sample - 10 ml. 
Preparation of a standard solution: 5 mg of gallic acid (t.n.) are placed in a 
volumetric flask with a capacity of 5 ml and dissolved in a mixture of water: methanol 
in a ratio (9:1) adjusted to the mark to obtain a standard solution of 1 mg/ml. 2 ml of the 
resulting solution is placed in a volumetric flask with a capacity of 20 ml, diluted to the 
mark with solvent and filtered. 
Preparation of the test solution: 1 ml of the liquid extract is placed in a volumetric 
flask with a capacity of 25 ml, dissolved with a mixture of water: methanol (9:1), 
adjusted to the mark with a solvent. 5 ml of the resulting solution is placed in a flask 
with a capacity of 20 ml and adjusted to the mark with the solvent and filtered. The 
retention time of 2.1 min of gallic acid in the liquid extract is comparable to the 
retention time of a standard sample. The content of gallic acid in the composition of the 
liquid extract is calculated by the following formula: 
Conclusions: developed a method for the qualitative and quantitative determination 
of gallic acid in the liquid extract "Extradent" by HPLC. It was revealed that the content 
of gallic acid was 0,2% . The relative error was 1,68 %.