Poster presentation
189
SYNTHESIS OF PARAMAGNETIC PARTICLES COATED WITH
SIO
2
LAYER FOR DNA/RNA ISOLATION FROM BIOLOGICAL
OBJECTS
A.A. Makhnev, A.B. Baimirzaev, E.A. Makhneva, D.R. Mansurov,
Kh.Z. Nasriddinov, O.B. Alimukhamedova, S.A. Ikramov, Sh.S. Asimov
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent
The synthesis of paramagnetic particles coated with a layer of SiO
2
is a promising
technology widely used in DNA/RNA isolation methods for
molecular diagnostics of
infectious and genetic diseases. The application of a paramagnetic sorbent makes it
possible to reduce the complexity of the stages of nucleic acid isolation and to automate
the process. The efficiency of DNA/RNA extraction by paramagnetic particles is
directly affected by the SiO
2
surface layer. Therefore, to study the sorption properties of
the paramagnetic sorbent, we synthesized magnetic particle A coated with one layer of
SiO
2
and magnetic particle B after three reactions of SiO
2
synthesis.
The synthesis of paramagnetic particles was carried out according to the following
protocol: 0.277 g of FeSO
4
•7H
2
O, 0.541 g of FeCl
3
•6H
2
O were dissolved in 5 ml of
distilled water, then 2 g of PEG-115 was added and the volume was adjusted to 10 ml
with water. Incubated at room temperature for 30 min on a shaker with a rotation speed
of 100 rpm.
Subsequently, 3 g of urea was added to the solution and incubated for
additional 1 hour at 50°C on a shaker with a rotation speed of 100 rpm. After
incubation, 1.5 ml of 30% ammonia solution is gradually poured into the solution with
stirring. As a result, Fe
3
O
4
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