Organizing co mmittee

BIOLOGICAL ACTIVITIES OF FLAVONOID SUM
ISOLATED FROM Crocus sativus 
 
D.A. Narbutaeva, Sh.R. Makhmudova, B.S. Okhundedaev, S.Z. Nishanbaev 
 
S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences 
of the Republic of Uzbekistan, 
e-mail: [e-mail]
 
Crocus sativus 
L. belongs to the 
Iridaceae
family, which is commonly cultivated in 
Europe and America. The commercial product and use of saffron comes from the dried 
stigmas, which impart a yellow color, bitter taste and intense aroma. Saffron is the most 
expensive spice in the world, combining useful properties. Possessing a rich chemical 
composition,
C. sativus 
L. has significant specificity and is used in medicine, 
cosmetology, cooking, as well as in the textile and perfume industries [1, 2]. 
At present, based on the special value and wide application of 
C. sativus
L., for the 
first time, work has been launched on its cultivation in the Republic of Uzbekistan. 
When the stigma-based saffron spice is obtained from the flowers of 
C. sativus
L., a 
huge number of petals are left as waste. 
The purpose of this work is to determine the biological activity of flavonoid sum 
isolated from the petals of 
C. sativus
L., which remain as a waste. 
Pharmacological studies have shown that the sum of flavonoids isolated from the 
petals of 
C. sativus
L. exhibits both antihypoxic activity (determined under conditions 
of acute normobaric hypoxic and hemic hypoxia) and antioxidant properties 
in vitro
(determined by the accumulation of malondialdehyde). Their antihypoxic and 
antioxidant activity was comparable to that of rutin, luteolin, and vitamin E. 
As can be seen from the results obtained, the sum of flavonoids from the petals of 
C. 
sativus
L., in comparison with rutin and luteolin, showed a pronounced antihypoxic 
activity, increasing the lifespan of animals in this case by 32.0-44.0%. Under conditions 
of hemic hypoxia, the increase in the lifespan of animals with the introduction of the 
studied drugs was 28.0–42.0%, and their inhibitory effect on the processes of lipid 
peroxidation 
in vitro
was from 60.0 to 75.0%, almost in the same range as the 
antioxidant effect. The most active was the sum of flavonoids from the petals of 
C. 
sativus
L., which was comparable with the activity of the reference drug, vitamin E 
(85.0%). 
The data obtained allow us to assert that the sum of flavonoids isolated from the petals 
of 
C. sativus
L. have a sufficiently antihypoxic and antioxidant effect. This sum of 
flavonoids is of practical interest for further in-depth studies of its pharmacological 
properties.