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nrDNA ITS2 Secondary Structure Prediction
Secondary structure of nrDNA ITS2 region for each species se-
quenced in the present study was explored using the minimum free
energy (MFE) program MFOLD (45) in GCG version 8.1 available at
http://rna.tbi.univie.ac.at/.
Results
Sequence Characteristics
The combined length of the entire ITS region (ITS1, 5.8S and ITS2)
from taxa sequenced in the present study ranged from 599-618
nucleotides (electrophenogram of one of he representative an-
nexed). The length of the ITS1 region and GC contents ranged from
201-203 nucleotides and 45-50% respectively, the 5.8S gene was
161-163 nucleotides long, the length of the ITS2 region and the GC
content ranged from 233-253 nucleotides and 48-51% respectively
[Table-6]. The aligned data matrix has a total number of 670 char-
acters (alignment annexed) of which 256 characters are constant,
255 characters were variable but parsimony-uninformative and 159
characters werre parsimony-informative. Insertions and deletions
(indels) were necessary to align the sequences. Indels ranged from
1 to 52 bp.
International Journal of Molecular Biology
ISSN: 0976-0482 & E-ISSN: 0976-0490, Volume 6, Issue 1, 2015
Phylogenetic Relationship Studies on the Genus Limonium Mill. Plumbaginaceae from Saudi Arabia Using its Sequences of Nuclear Ribosomal
DNA
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