Tomat dengan menggunakan solven campuran

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Temperatur 40

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.301) – 0.0926

0.0613

= 3.4 mg/100gr

Kadar lycopene yg terekstrak = 3.4 mg/100gr

Temperatur 50

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.30) – 0.0926

0.0613

= 3.88 mg/100gr

Kadar lycopene yg terekstrak = 3.88 mg/100gr

Temperatur 60

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.309) – 0.0926

0.0613

= 3.54 mg/100gr

Kadar lycopene yg terekstrak = 3.54 mg/100gr

Temperatur 70

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.313) – 0.0926

0.0613

= 3.6 mg/100gr

Kadar lycopene yg terekstrak = 3.6 mg/100gr



Temperatur 80

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.2145) – 0.0926

0.0613

= 1,99 mg/100gr

Kadar lycopene yg terekstrak = 1,99 mg/100gr

d.

Perbandingan F/S (1:4)

Temperatur 30

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.301) – 0.0926

0.0613

= 3.41 mg/100gr

Kadar lycopene yg terekstrak = 3.41 mg/100gr

Temperatur 40

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.33) – 0.0926

0.0613

= 3.87 mg/100gr

Kadar lycopene yg terekstrak = 3.87 mg/100gr

Temperatur 50

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.3659) – 0.0926

0.0613

= 4.36 mg/100gr

Kadar lycopene yg terekstrak = 4.49 mg/100gr

Temperatur 60

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.36) – 0.0926

0.0613

= 4.46 mg/100gr

Kadar lycopene yg terekstrak = 4.46 mg/100gr

Temperatur 70

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.369) – 0.0926

0.0613

= 4.51 mg/100gr

Kadar lycopene yg terekstrak = 4.51 mg/100gr

Temperatur 80

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.366) – 0.0926

0.0613

= 4.47 mg/100gr

Kadar lycopene yg terekstrak = 4.47 mg/100gr

e.

Perbandingan F/S (1:5)

Temperatur 30

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.331) – 0.0926

0.0613

= 3,38 mg/100gr

Kadar lycopene yg terekstrak = 3,38 mg/100gr

Temperatur 40

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.335) – 0.0926

0.0613

= 3.89 mg/100gr

Kadar lycopene yg terekstrak = 3.89 mg/100gr

Temperatur 50

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.33) – 0.0926

0.0613

= 3.87 mg/100gr

Kadar lycopene yg terekstrak = 3.87 mg/100gr

Temperatur 60

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.315) – 0.0926

0.0613

= 3.63 mg/100gr

Kadar lycopene yg terekstrak = 3.63 mg/100gr

Temperatur 70

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.302) – 0.0926

0.0613

= 3.57 mg/100gr

Kadar lycopene yg terekstrak = 3.57 mg/100gr

Temperatur 80

o

C

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.296) – 0.0926

0.0613

= 3,33 mg/100gr

Kadar lycopene yg terekstrak = 3,333 mg/100gr

Kadar lycopene pada perbandingan F/S(1:4) dan suhu 70

o

C

Waktu operasi 30 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.353) – 0.0926

0.0613

= 4,25 mg/100gr

Kadar lycopene yg terekstrak = 4,25 mg/100gr

Waktu operasi 40 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.3617) – 0.0926

0.0613

= 4,39 mg/100gr

Kadar lycopene yg terekstrak = 4,39 mg/100gr

Waktu operasi 50 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.3647) – 0.0926

0.0613

= 4,44 mg/100gr

Kadar lycopene yg terekstrak = 4,44 mg/100gr

Waktu operasi 60 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.368) – 0.0926

0.0613

= 4,5 mg/100gr

Kadar lycopene yg terekstrak = 4,5 mg/100gr

Waktu operasi 70 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.370) – 0.0926

0.0613

= 4,54 mg/100gr

Kadar lycopene yg terekstrak = 4,25 mg/100gr

Waktu operasi 80 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.376) – 0.0926

0.0613

= 4,63 mg/100gr

Kadar lycopene yg terekstrak = 4,63 mg/100gr

Waktu operasi 90 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.4076) – 0.0926

0.0613

= 5,14 mg/100gr

Kadar lycopene yg terekstrak = 5,14 mg/100gr

Waktu operasi 100 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.411) – 0.0926

0.0613

= 5,2 mg/100gr

Kadar lycopene yg terekstrak = 5,2 mg/100gr

Waktu operasi 110 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.416) – 0.0926

0.0613

= 5,29 mg/100gr

Kadar lycopene yg terekstrak = 5,29 mg/100gr

Waktu operasi 120 menit

Kadar lycopene= y = 0.0613x + 0.0926

x = y – 0.0926

0.0613

= (0.423) – 0.0926

0.0613

= 5,4 mg/100gr

Kadar lycopene yg terekstrak = 5,4 mg/100gr

Dalam proses ekstraksi lycopene dari buah tomat ada pengaruh dari perbandingan

antara feed dengan solven, serta kenaikan suhu terhadap lycopene yang terekstrak

sehingga operasi terbaik adalah operasi pada kondisi maksimum pada

perbandingan feed dan solven 1:4 pada suhu operasi 70˚C dan selama 90 menit.

Pada kondisi ini lycopene yang terekstrak sebesar 5,14 mg/100gram jus buah

tomat.

Lampiran II : Kurva Standart Lycopene

Deret Standart dan absorban/pada 470 nm :

2

4

0.2084

0.3411

0.467

0.5888

0.698

kurva standart Licopene

y = 0.0613x + 0.0926

= 0.9987

0.2

0.4

0.6

0.8

15

Konsentrasi ( mg/100gr )

a

b

s

o

rb

a

n

s

i

Lampiran III : Gambar

Gambar 1. Buah tomat

Gambar 2. Proses Pengehalusan buah

tomat (juice)

Dilakukan pemilihan buah tomat

Gambar 3. Gambar alat utama ekstraksi Gambar 4. Proses

memasukan pelurut kedalam alat

Gambar 5. Proses pemasukan juice tomat

ke dalam alat utama ekstraksi

Gambar 6. Hasil ekstraksi

Gambar 8. Spektrofotometer

Gambar 7. Proses pemisahan ekstrak dan rafi

nat

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