Transplantologiya 2014

Ischemia/reperfusion in a rat model of strangulated intestinal obstruction.

According to the experimental study protocol, a prolonged anesthesia was administered to animals intravenously in vena caudalis using a mixture containing 0.5 ml of 3.5% Zoletil® 100 solution (Zoletil® 100, Virbac Sante Animale, France) and 0.1 ml of Rometar solution (Rometar, Bioveta, Czech Republic). Following induction of anesthesia, the intended operative site was prepared by removing fur and decontaminated with 70% alcohol solution. In anesthetized rats, the abdomen was opened via a midline laparotomy incision. The distal ileum loop (4-5 cm long) and adjacent mesentery with blood vessels were pulled out through the laparotomy wound. Strangulation of the bowel loops with blood supplying vessels was induced by applying an occlusion device consisted of a silk ligature fixed inside the tube of 1 mm in diameter (Fig. 1).

Figure 1. Strangulation of the ileum loops with blood supplying vessels using the occlusion device that consists of a silk ligature fixed inside the tube.

The ischemia of the distal ileal loop and mesenteric vessels was maintained for 60 minutes. After 60 minutes, the occlusion device was removed off the ischemic ileal loop. The restoration of blood flow in the loop was verified by a changing coloration of the strangulated intestinal portion and a visible pulsation in the mesenteric vessels. After the blood flow was restored, the abdominal cavity was closed tightly using a Policon Poliamide sterile surgical suture (Policon, Tonzos 95, Bulgaria). After the strangulation had been resolved, the reperfusion period made 120 minutes.