Transplantologiya 2014

Experimental study protocol

Animals were randomly allocated into the following groups:

Group I (sham operated animals). The animals in this group (n=5) were subjected to a surgical procedure that comprised opening the abdomen to isolate an intestinal loop for a period of 60 minutes without strangulation.

Group II (I/R). The animals (n=5) were subjected to a surgical procedure that involved opening the abdomen to create an ischemia/reperfusion (I/R) model by inducing a strangulated small bowel obstruction. The ileum loop with mesenteric vessels was occluded for 60 minutes that was followed by a 120-minute reperfusion period.

Group III (Prx VI). The animals (n=5) received 10 mg/kg of human recombinant Prx VI administered intravenously at 15 minutes before intestinal loop ischemia.