Microbial & clinical studies have revealed that, periodontitis is caused by bacteria, which inhabitat periodontal pocket & bacterial colonization may play an important role in pathogenesis of the disease.1,2
A high proportion of progressing periodontal infection in adults have been associated with presence or elevated levels of Porphyromonas gingivalis & that it might not be a normal inhabitant of periodontally healthy subjects1,2,3. Facilitating detection of this pathogen would be a major benefit in periodontal risk assessment4.
The Culture & DNA hybridization is currently available for testing the presence of periodontal pathogens , the culture technique is advantageous in detecting the antibiotic sensitivity of different pathogens in order to determine the appropriate therapy & disadvantage being, time consuming for growth & isolation of specific organisms, survival of bacteria during transportation to laboratory is questionable. DNA probes is used for identification of specific pathogens regardless of their viability, disadvantage being lesser limit of sensitivity.2,5
In recent years, based on molecular biology techniques ,Polymerase chain reaction(PCR) has been developed which is simple, sensitive, less time consuming technique. PCR replicates a fragment of RNA or DNA for the detection of specific periodontopathic bacteria. It utilizes a pair of primer, that are selected from opposing strands of a gene which is specific to a particular organism.2,4,5
The purpose of the present study is to detect Porphyromonas gingivalis using Polymerase chain reaction(PCR) in periodontally healthy subjects & chronic periodontitis patients.
Objectives of the study:
The objective of this study is to detect Porphyromonas gingivalis in subgingival plaque samples in healthy subjects & chronic periodontitis patients using polymerase chain reaction technique .
To assess the relationship of Porphyromonas gingivalis in health & chronic periodontitis .
The following observations will be made
a).Presence of Porphyromonas gingivalis in 10 clinically healthy periodontium.
b) Presence of Porphyromonas gingivalis in 10 chronic generalized periodontitis patients from subgingival plaque samples obtained from a single site of pocket depth measuring ≥5mm per patient.
Materialsand Methods 1. Source of data: Systemically healthy patients visiting the department of Periodontics, Krishnadevaraya College Of Dental Sciences & Hospital, Bangalore, will be selected.
-Chronic generalized periodontitis patients (test group)
2. Method of collection of data: Sample size: 20 patients attending the out patient department of periodontics, KCDS, Bangalore and satisfying inclusion and exclusion criteria will be taken for the study
Control group – 10 systemically healthy patients with clinically healthy periodontium and
Test group -10 systemically healthy patients with history of chronic generalized periodontitis , satisfying the inclusion criteria .
1 subgingival plaque sample from one site of pocket depth measuring ≥5mm from each chronic generalized periodontits patients.