Reviews: 7 Publications: 72



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Product Datasheet

Caspase-3 Antibody  - Active

AF835-SP

Unit Size: 25 ug



Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from 

    Reviews: 7

       

    Publications: 72

    Protocols, Publications, Related Products, Reviews, Research Tools and Images at:

    www.novusbio.com/AF835

    Updated 5/10/2016 v.20.1



AF835-SP

Caspase-3 Antibody - Active



Product Information

Unit Size

25 ug


Concentration

LYOPH mg/ml



Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 

reconstitution.



Clonality

Polyclonal



Preservative

Trehalose



Reconstitution Instructions

Reconstitute at 0.2 mg/mL in sterile PBS.



Isotype

IgG


Purity

Immunogen affinity purified



Buffer

Lyophilized from a 0.2  m filtered solution in PBS with Trehalose. *Small pack 



Product Description

Host

Rabbit


Gene ID

836


Gene Symbol

CASP3


Specificity/Sensitivity

Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. 



Immunogen

KLH-coupled human Caspase-3 synthetic peptide

CRGTELDCGIETD

Accession # P42574 



Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne 

brand.

Product Application Details

Applications

Immunocytochemistry



Recommended Dilutions

Immunocytochemistry 5-15 ug/mL

Page 1 of 4 v.20.1 Updated 5/10/2016


Images

leukemia cell line stimulated with staurosporin using Human/Mouse 

stained using the NorthernLights

557-conjugated Anti-Rabbit IgG 

NL004

) and counterstained with 



DAPI (blue). View our protocol for 

Fluorescent ICC Staining of Cells on 

Coverslips

.

Caspase‑3 was detected in immersion fixed anti-FAS treated 



Human/Mouse Active Caspase‑3 Antigen Affinity-purified 

Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. 

Cells were stained (red) and counterstained (green). View our protocol 

for 


Fluorescent ICC Staining of Cells on Coverslips

.

Page 2 of 4 v.20.1 Updated 5/10/2016



Publications

Lindgren G, Ekblad L, Vallon-Christersson J, Kjellen E, Gebre-Medhin M, Wennerberg J Erythropoietin suppresses 

the activation of pro-apoptotic genes in head and neck squamous cell carcinoma xenografts exposed to surgical 

trauma. BMC Cancer, 2014;14(0):648. 2014 [PMID: 25182342] (IHC Not Specified, , Mouse)

Levet S, Ouarne M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, 

Feige J, Tillet E, Bailly S BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus. Proc Natl Acad 

Sci U S A, 2015;112(25):E3207-15. 2015 [PMID: 26056270] (IHC Not Specified, , Mouse)

Scott C, Cha K, Rao R, Liddle C, George J, Gunton J Hepatocyte-specific deletion of ARNT (aryl hydrocarbon 

Receptor Nuclear Translocator) results in altered fibrotic gene expression in the thioacetamide model of liver injury. 

PLoS ONE, 2015;10(3):e0121650. 2015 [PMID: 25812120] (IHC Paraffin-embedded, , Mouse)

Quereda V, Porlan E, Canamero M, Dubus P, Malumbres M An essential role for Ink4 and Cip/Kip cell-cycle inhibitors 

in preventing replicative stress. Cell Death Differ, 2015;23(3):430-41. 2015 [PMID: 26292757] (IHC Paraffin-

embedded, , Mouse)

Jacob F, Yonis A, Cuello F, Luther P, Schulze T, Eder A, Streichert T, Mannhardt I, Hirt M, Schaaf S, Stenzig J, Force 

T, Eschenhagen T, Hansen A Analysis of Tyrosine Kinase Inhibitor-Mediated Decline in Contractile Force in Rat 

Engineered Heart Tissue. PLoS ONE, 2016;11(2):e0145937. 2016 [PMID: 26840448] (IHC Paraffin-embedded, , Rat)

Declercq , Annelies, Chiers , Koen, Van den Broeck , Wim, Dewulf , Jeroen, Eeckhaut , Venessa, Cornelissen , 

Maria, Bossier , Peter, Haesebrouck , Freddy, Decostere , Annemie Interactions of highly and low virulent 

Flavobacterium columnare isolates with gill tissue in carp and rainbow trout. Vet Res, 2015;46(0):25. 2015 [PMID: 

25889257] (IHC Paraffin-embedded, , Fish - Cyprinus carpio (Carp))

Wong J, Chen L, Huang Y, Sehba F, Friedel R, Zou H Attenuation of Cerebral Ischemic Injury in Smad1 Deficient 

Mice. PLoS ONE, 2015;10(8):e0136967. 2015 [PMID: 26317208] (IHC Frozen, , Mouse)

Amelkina O, Zschockelt L, Painer J, Serra R, Villaespesa F, Braun B, Jewgenow K Apoptosis-Related Factors in the 

Luteal Phase of the Domestic Cat and Their Involvement in the Persistence of Corpora Lutea in Lynx. PLoS ONE, 

2015;10(11):e0143414. 2015 [PMID: 26599641] (IHC - paraffin, , Feline)

Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R Crim1 maintains retinal 

vascular stability during development by regulating endothelial cell Vegfa autocrine signaling. Development, 2014;141

(2):448-59. 2014 [PMID: 24353059] (IHC Not Specified, , Mouse)

Sharma S, Chintala N, Vadrevu S, Patel J, Karbowniczek M, Markiewski M Pulmonary alveolar macrophages 

contribute to the premetastatic niche by suppressing antitumor T cell responses in the lungs. J Immunol, 2015;194

(11):5529-38. 2015 [PMID: 25911761] (Flow Cytometry, , Mouse)

Sun H, Lou Y, Porturas T, Morrissey S, Luo G, Qi J, Ruan Q, Shi S, Chen Y Exacerbated experimental colitis in 

TNFAIP8-deficient mice. J Immunol, 2015;194(12):5736-42. 2015 [PMID: 25948814] (IHC Paraffin-embedded, , 

Mouse)


Sesen J, Dahan P, Scotland S, Saland E, Dang V, Lemarie A, Tyler B, Brem H, Toulas C, Cohen-Jonathan Moyal E, 

Sarry J, Skuli N Metformin inhibits growth of human glioblastoma cells and enhances therapeutic response. PLoS 

ONE, 2015;10(4):e0123721. 2015 [PMID: 25867026] (IHC - Not specified, , Mouse)

More publications at 

http://www.novusbio.com/AF835

Page 3 of 4 v.20.1 Updated 5/10/2016



Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. 

Primary Antibodies are guaranteed for 1 year from date of receipt.

For more information on our 100% guarantee, please visit 

www.novusbio.com/guarantee

Earn gift cards/discounts by submitting a review: 

www.novusbio.com/reviews/submit/AF835

Earn gift cards/discounts by submitting a publication using this product: 

www.novusbio.com/publications

Novus Biologicals USA

8100 Southpark Way, A-8

Littleton, CO 80120

USA


Phone: 303.730.1950

Toll Free: 1.888.506.6887

Fax: 303.730.1966

novus@novusbio.com



Novus Biologicals Canada

461 North Service Road West, Unit B37

Oakville, ON L6M 2V5

Canada


Phone: 905.827.6400

Toll Free: 855.668.8722

Fax: 905.827.6402

canada@novusbio.com



Novus Biologicals Europe

19 Barton Lane

Abingdon Science Park

Abingdon, OX14 3NB, United Kingdom

Phone: (44) (0) 1235 529449

0800 37 34 15

Fax: (44) (0) 1235 533420

info@bio-techne.com



General Contact Information

www.novusbio.com

Technical Support: technical@novusbio.com

Orders: orders@novusbio.com



General: novus@novusbio.com

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