Organizing co mmittee

 
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Poster presentation 
98 
METABOLITES’ COMPOSITION OF Stachys hissarica 
 
E.A. Mukhammadiev, N.Z. Маmаdalieva 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: [e-mail]
 
Stachys 
is a plant genus, one of the largest in the Lamiaceae (mint) family. Several 
Stachys
species have been used in various ethnomedicines for thousands of years. 
Stachys
have been used in traditional medicine as astringent, wound healing, 
antidiarrheal, antinephritic, and anti-inflammatory agents. 
Stachys hissarica
Regel (local name is Hisor quddusi) is a perennial species that 
occurs in the regions of Tashkent, Samarkand, and Surkhandarya. This plant is an 
endemic of the Pamir-Alay, grows in mountainous areas, in rocky places. Information 
on the chemical composition of the 
Stachys hissarica
plant is not available in the 
literature. 
The aerial parts of 
S. hissarica
(1.9 kg) were collected from the Tashkent region. The 
dried plant was extracted with methanol. As a result, 270 g of crude methanolic extract 
was obtained. To precipitate the chlorophyll in the extract, the dark extract was mixed 
with 60% ethyl alcohol and placed in a cold place. At the next step (after the 
chlorophyll was completely precipitated and separated), the extract was divided into 
chloroform (32 g), ethylacetate (5 g) and butanol (84 g) fractions. 
Butanol fraction of 
S. hissarica
was subjected to the column chromatography with 
silicagel and eluted with solvents such as chloroform, chloroform: methanol (increasing 
methanol to 20%). For TLC, as a mobile phase, the system: tetrahydrofuran-toluene-
1mM solution of trifluoroacetic acid in methanol-water (16:8:2:1) was used. Results 
showed that butanol extract is rich in iridoids, flavonoids, phenylethanoids, and 
glycosides. Research studies are continuing on the isolation of pure compounds.