Poster presentation
160
1-O-METHYLEMODIN AND STIGMASTEROL COMPOUNDS
FROM THE UNDERGROUND PART OF RUMEX PAMIRICUS
G.D. Shermatova
1
, K.A. Eshbakova
2
, Sh.Sh. Sagdullaev
2
, B.S. Mirzaev
1
1) “TIIAME” National Research University, 100000, Tashkent, 39, Kari-Niyaziy str.,
e-mail: gdshermatova@gmail.com
2) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent
The basis of the technology for the isolation of 1-
O
-Methylemodin and Stigmasterol
(Fig.1) compounds is the extraction of raw materials with various organic solvents
followed by chromatographic purification. Classical extraction methods (percolation
and maceration) are time-consuming and laborious.
The herb
Rumex pamiricus
Rech. f. belongs to the family of Polygonaceae and there
are over 250 types on the earth and 16 types in Uzbekistan.
The roots of the herb
Rumex pamiricus
dried at room temperature, in shade. The
pounded herb roots were first subjected to extraction in chloroform, then three times in
70% acetone hydrous solution. The acetone extract was distilled under vacuum; the
remaining water solution was subjected to extraction with ethyl acetate. Ethyl acetate
extracts were collected and were dehydrated by adding anhydrous salt Na
2
SO
4
. The
dehydrated extract was filtered, its concentration increased under vacuum, the total
phenols were precipitated by adding pure hexane to the condensed extract. The created
precipitate was washed, and filtered and the extracted total phenols of chloroform and
ethyl acetate fractions constituted 3.4% of the herb dry weight.
The chloroform fraction subjected with column chromatography on KSK silica gel,
eluted with a mixture of extraction benzene–ethyl acetate: (50:1, 40:1, 30:1, 20:1 and
10:1). The structure of 1-
O
-methylemodin and stigmasterol was established on the basis
of the analysis of the data of MS (Mass spectrometry),
1
Н and
13
С NMR spectra
(Nuclear Magnetic Resonance), and of the DEPT (Distortionless Enhancement of
Polarization Transfer), HSQC (Heteronuclear Single Quantum Coherence) and HMBC
(Heteronuclear Multiple Bond Correlation) experiments. Qualitative analyses of major
phenolics by TLC (Thin Layer Chromatography) analysis were also evaluated.
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