Organizing co mmittee

1
was obtained.
Triterpene glycoside (
1
) was obtained from 
A. gypsophiloides
. The structure of this 
triterpenoid was elucidated as gypsogenin 3-
O
-α-L-arabinopyranosyl-(1→3)-[β-
D
-
galactopyranosyl-(1→2)]-β-
D
-glucuronopyranoside by comprehensive 1 D and 2 D 
NMR experiments, as well as high-resolution mass spectrometry. This compound was 
previously isolated from 
Gypsophila trichotoma
. 
1 
Triterpenes comprise one of the most interesting groups of natural products because 
of their high potential as pharmacological agents. On the basis of mass spectra and 
NMR data, one known saponin was identified for the first time in the 
A. gypsophiloides
roots.

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Poster presentation 
177 
EVALUATION QUALITY OF THE DRY EXTRACT 
OBTAINED ON THE BASIS СELANDINE 
N.B. Abdunazarova, E.S. Karieva 
Tashkent Pharmaceutical Institute, Tashkent, Oybek, 45 
Celandine - 
Chelidonium majus L
. - belongs to the 
Papaveraceae
family. Celandine 
grows on cool lands, among shrubs, on forest edges, in ravines, in places, in settlements, 
in gardens and fields. The celandine plant is found in Europe, Altai, Central Asia, 
Siberia and the Far East. It grows in small groups, but in some cases, it can also form 
thickets. Celandine loves moist and fertile soils. 
The celandine plant exhibits such pharmacological effects as antimicrobial, antiviral, 
anti-inflammatory, antispasmodic. Dry extracts obtained from medicinal plants are used 
for new drugs. Based on this, a dry extract was obtained from grass celandine. 
The resulting extract was evaluated according to the following parameters: 
appearance, exposure, weight loss on drying, moisture content, amount of heavy metals, 
microbiological purity and quantitative determination of the amount of alkaloids. These 
studies were identified using the methods presented in the following regulatory 
documents: "UzR SF I, RF SF XIV, IPA.1.4.1.21 "Extracts", European Pharmacopoeia, 
9
th
edition "Extracts"". 
Based on the results obtained, the extract is a brown powder with a specific odor. Its 
authenticity was determined by carrying out quality tests on the main influencing 
substance, i.e. alkaloids. Under the influence of 10% sulfate and silicon tungstic acid, a 
white-yellowish precipitate precipitated. 
According to the requirements of regulatory documents, during drying, the mass loss 
should not exceed 5%. In the analyzed dry extract, this index was 3.92%. 
The color of the prepared solution for determining the amount of heavy metals, the 
color of the solution is not darker than the color of the standard solution, that is, the dry 
extract, which is also analyzed for this indicator, corresponds the requirements of 
Normative Documents. 
No bacteria were found in 1 g of dry extract of 
Pseudomonas aeruginosa
, 
Staphylococcus aureus, Escherichia coli
. It was found that the total number of aerobic 
microorganisms is no more than 10
4
КОЕ, yeast and mold fungi - from 10
2 
КОЕ, bile-
resistant enterobacteria - no more than 10
2 
КОЕ. 
To determine the amount of accumulation of alkaloids in relation to the chelidonin 
contained in the dry extract of celandine, the method presented in the pharmacopoeia 
was used: this index was 1.3%. 
According to the results of the studies, the dry extract of the celandine according to 
the indicators corresponds to the qualitative and quantitative requirements of the current 
normative documents. In the future, the dry extract is planned to be used as the basis for 
obtaining a wound healing and anti-inflammatory ointment.