Organizing co mmittee

DETERMINATION OF MACRO- AND MICRO-ELEMENTS IN 
PERSIMMONS BY THE METHOD OF MASS-SPECTROMETRY 
WITH INDUCTIVELY-COUPLED PLASMA 
 
A.A. Ibragimov, A.Sh. Toyiraxon, Sh.Sh. Isakova 
 
Ferghana State University 
 
Persimmon is a subtropical tree or shrub belonging to the genus Diospyros of the 
Ebenacea family. About 500 species are found in tropical and subtropical regions. The 
palm tree is grown in China, Japan, the Mediterranean countries, Australia, the USA, the 
Caucasus, Central Asia and Uzbekistan. Tree 8-12 m high, trunk rounded or pyramidal. 
Lives 100-400 years, sometimes more, but good fruits bear fruit up to 50-60 years. 
Tolerates cold up to 15-20°C, photophilous plant. The leaf is large, ovoid, dark green 
above, pubescent on the reverse side, arranged in a row, turns red before falling off. 
Persimmon leaves for the study were collected in the Baghdad district of the Fergana 
region. 30 ml of concentrated nitric acid was added to the crushed leaves in a flask and 
kept for 30 min until a clear solution was obtained. Then the resulting solutions were 
filtered into flasks with a capacity of 100 ml and topped up with distilled water to the 
mark. The mineral composition was determined by inductively coupled plasma mass 
spectrometry on an ICP-MS AT 7500 instrument. 
In the course of studying the mineral composition of persimmon leaves by 
inductively coupled plasma mass spectrometry, the content of 44 elements was 
analyzed. Of these, the content of 40 elements was determined. The content of the 
elements decreases in the following order Ca 

K 

Mg 

Fe 

Si 

Mn 

S 

Al 

Sr 

B 

Na 

Ti 

Ba 

Zn 

Rb 

Sn 

Ni 

Li 

Cu 

As 

Cr 

Mo 

Pb 

Hg 

Se 

V 

Ga 

Zr 

Co 

U 

Sb 

Cd 

Cs 

Nb 

Re 

W 

Ag = Ge 

Ta 

BeIn the leaves, 
the total content of elements is 35750.313 mg/l. The leaves contain 5 macronutrients. It 
has been established that the amount of macronutrients in persimmon leaves is for Ca 
23545.653 mg/l, K 6284.906 mg/l, Mg 3843.511 mg/l, S 142.312 mg/l and Na 30.310 
mg/l. The following trace elements were found in the leaves Si, Al, Fe, B, Mn, Co, Ni, 
Cu, Zn, Sr, Ba, Rb, Ga, Cr, Ge, Se, Li, Be, Ti, Sb, Sn, Sb, Cs, Ba, Li, Mo, Ag, Ta V, Re, 
V and U. The largest amount of microelements in the leaves are: Fe 990.545 mg/l, Si 
347.766 mg/l, Mn 214.732 mg/l, Al 122.139 mg/l, Sr 109.934 mg/l and B 75.146 mg/l. 
The remaining elements have a concentration of 0.001-16.622 mg/l. Of the toxic 
elements, arsenic, cadmium, mercury and lead were found. The content of toxic 
elements is 0.012-1.241 mg/l.
Analysis of the macro- and microelement composition of 
persimmon leaves shows a tendency for the amount of the element to decrease with an 
increase in atomic mass. According to the results of the study, persimmon leaves are a 
rich source of calcium and can be used as ingredients in various nutritional 
supplements. 

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Poster presentation 
85