Organizing co mmittee

 
 

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Poster presentation 
100 
METABOLOMIC PROFILING AND BIOLOGICAL ACTIVITIES 
OF Ferula foetida USING UHPLC-MS AND GC-MS ANALYSIS 
N. Z. Mamadalieva
1
, O. K. Khojimatov
2
, L. A. Wessjohann
3
 
1) Institute of Botany of the Academy Sciences of Uzbekistan, DurmonYuli Str 32, 
Tashkent 100125, Uzbekistan
; 
2) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
3) Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany. 
e-mail: [e-mail]
 
The genus 
Ferula 
has a wide distribution all over Central and South-West Asia 
(especially Uzbekistan, Iran, and Afghanistan), the far-East, North India, and the 
Mediterranean, and some are distributed in desert areas. There are 114 species of 
Ferula
in Central Asia and about 60 of them in Uzbekistan. In Uzbekistan 
Ferula
species grow 
in sandy deserts, hills, mountains, and foothills of the country, in Tashkent, 
Surkhandarya, Kashkadarya, Jizzakh, Navoi, Bukhara regions and the Republic of 
Karakalpakstan. The majority of the 
Ferula
plants have a pungent odor and can be used 
for different purposes. 
The oleo-gum-resin of 
Ferula foetida 
(Bunge) Regel is a popular food and herbal 
supplement. The full complement of bioactive compounds has yet to be elucidated, a 
step necessary in order to explain its nutraceutical and medicinal use. Here, untargeted 
metabolic profiling techniques were utilized to help gain a broader insight into oleo-
gum-resin of 
F. foetida
chemical composition. The technical aims of the project focused 
on the development of analytical methods for metabolic profiling of oleo-gum-resin 
obtained from 
F. foetida
species. The metabolite profiling of the species carried out 
using rapid and sensitive chromatographic (UHPLC, GC) and mass spectrometric (MS) 
methods.
UHPLC-MS metabolite profiling of the resin obtained from 
F. foetida 
identified high 
levels of coumarins and their derivatives, flavonoids, fatty acids. Metabolites identified 
for 
F. foetida 
included several metabolites, such as foetisulfide A, rivulobirin G, 
quercetin, microlobin, 1-acetate-5-isovalerate of lapiferol, 1-acetate-6-isovaleriat 
ferulinkiol, ethyl myristate, 
cis
-octadecenoic acid, etc. In the oleo-gum-resin of 
F. 
foetida
, the major volatile compounds were bis (1-methylpropyl) disulfide, 2-
(isopropyldisulfanyl) butane, β-ocimene and α-pinene. 
In addition, biological effects of the oleo-gum-resin of 
F. foetida 
was evaluated. The 
results of anthelmintic, antifungal, and cytotoxic assays demonstrated that the oleo-
gum-resin was not toxic in 
in vitro
assays.