Organizing co mmittee

LOW MOLECULAR METABOLITES OF FUNGI. 
13,22-DIMETOXYSTACHIBOTRIN FROM Stachybotrys chartarum 
 
S.F. Aripova
1
, L.S. Kamalov
2
, S. Tojieva
2
, I. Nakhatov
2 
 
1) S.Yu. Yunusov Institute of Chemistry of Plant Substances. AS RUz, 
2) Karshi State University, Uzbekistan. 
e-mail: [e-mail] 
As a result of studies carried out in our country, information was obtained on the 
distribution of fungi of the genus 
Stachybotrys,
their morphology, ultrastructure, 
cytology, biotechnology of cultivation on cellulose-containing nutrient media, ensiling 
of such solid plant waste as guzapoya, rice straw, corn stalks. 
Stachybotrys chartarum
produces a number of low molecular weight compounds. A technique has been 
developed for the isolation and separation of the sum of extractive substances - waste 
products of the fungus 
Stachybotrys chartarum
grown under laboratory conditions on 
various nutrient media. 
Cause and formulation of the problem, purpose of the work:
The purpose of the 
study is the selection of active local strains of micromycetes, forming 13,22-
dimethoxystachibotrin and stachibotral, the study of their morphological cultural 
properties, the creation of a complex biological product based on promising local strains 
that protect against fungal phytopathogens. 
Results/conclusions:
Studied 3 strains of fungi of the genus 
Stachybotrys 
isolated 
from the rhizosphere of agricultural crops of serozem soils of Tashkent region and 
available in the collection of the laboratory. As a result of studying the cultural 
morphological features, the identification of the strains of 
Stachybotrys chartarum
was 
carried out and it was recommended to cultivate in a nutrient medium supplemented 
with 2% sucrose and 2% molasses. As a result of screening the selected 
microorganisms, new local strains of 
Stachybotrys chartarum
were obtained and 
identified with antimicrobial activity, antagonistic properties against phytopathogens 
and forming a wide range of 13,22-dimethoxystachibotrin and stachibotral in a liquid 
culture medium.
The optimal nutrient medium was selected for the selected strains (the influence of 
various carbon sources) and the cultivation conditions for the production of the greatest 
amount of 13,22-dimethoxystachibotrin and stachibotral under submerged conditions.
An analysis of the spectral data of the isolated substances and the products of their 
chemical transformations showed that they are all new, based on the same skeleton, 
consisting of 23 carbon atoms, which is a condensed system of the sesquiterpenoid of 
dryman with benzofuran.