Organizing co mmittee

2-4
) of bicyclic quinazolones in good yields: 
The structure of the synthesized sulfonylureas (
2-4
) was proved by IR and NMR 
spectra. 
Reference 
1. Elmuradov B. J. Synthesis, chemical transformations and biological activity of 
condensed benzo- and thienopyrimidine-4-ones. 
Dissertation for the scientific degree of 
DSc in Chemical Sciences, 
Tashkent, 2019, P.200. 

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Poster presentation 
249 
SYNTHESIS OF BIOACTIVE COMPOUNDS OF LOCAL 
BACTERIA AT DIFFERENT CONCENTRATIONS OF HEAVY 
METAL IONS 
A. Usmonkulova
1
, G. Kadirova
1
, M. Narmukhamedova
1
, D. Turaqulova
2
 
1) Institute of Microbiology of the Academy of Sciences, Tashkent, Uzbekistan; 
2) Tashkent State Technical University;
e-mail: [e-mail] 
Cadmium (Cd) and nickel (Ni) are among the most toxic metals and high levels of 
Cd and Ni in soil have been observed in several studies to slow down plant growth, 
mainly biomass, chlorophyll content and photosynthetic properties. Beneficial soil 
bacteria are promising targets for reducing Cd and Ni toxicity and increasing plant 
tolerance to heavy metal stress.
In this study stimulating properties of plant productivity under conditions of heavy 
metal stress were studied. Exopolysaccharid, Auxin and gibberellin synthesis at 
different concentrations of heavy metal ions were determined.
Pseudomonas 
aeruginosa, 
Enterobacter 
cloacae, 
Bacillus 
licheniformis, 
Enterobacter ludwigii, Bacillus simplex
strains were used as research objects. Bacteria 
were grown for 3-7 and 14 days in peptone broth added in concentrations up to added 
57.3 mg/l, 95.7 mg/l and 191.4 mg/l and 2.44 mg/l, 4.1 mg/l and 8.2 mg/l to the medium 
of heavy metals (NiSO
4
× 7H
2
O and CdCl
2
× 2.5 H
2
O). Indole-3 acetic acid (IAA) and 
gibberellin synthesis of bacteria was periodically determined for 3, 7 and 14 days 
according to the spectrophotometric method recommended by Berrios et al. Synthesis of 
exopolysaccharides by the strains was determined for 3, 7 and 9 days. 
Pseudomonas aeruginosa
synthesized auxin 4, 4.8, 5.3 times higher amount 
compared to control and 
Enterobacter ludwigii
, 
Bacillus licheniformis, Bacillus
simplex 
synthesized auxin in equal amount compared to the control at different concentrations of 
Cd cation (2.4, 4.1, 8.2 mg/l). Gibberellin synthesis in the strains decreased with 
increasing concentration of heavy metals (Cd(II) and Ni (II)). 
Pseudomonas aeruginosa
synthesized 375 μg/ml gibberellin in the control and 390 μg/ml in the Ni
2+
95.7 mg/ml 
concentration on the 7
th
day of cultivation, and 
Enterobacter ludwigii
375 μg/ml in the 
control and 380 μg/ml in the Cd
2+
4.2 mg/l concentration on the 3
rd
day of cultivation. 
During experiments aimed at determining the synthesis of exopolysaccharides in 
Bacillus atropheus
(4) and 
Enterobacter ludwigii
(11) strains grown for 14 days at 
concentration of 8.2 and 24.6 mg/l Cd(II) cation, EPS synthesis on the 7
th
and 14
th
days 
of cultivation was increased to 26, 28 mg/l and 6, 11 mg/l. 
Pseudomonas aeruginosa
(18) strain produced 78 mg/l EPS at the concentration of 24.6 mg/l Cd(II) cation on the 
7
th
day of cultivation. 
Using microorganisms tolerant of high concentrations of heavy metals and their 
activity, bioremediation of ambience contaminated with heavy metals is possible. 
Increased synthesis of phytohormones and exopolyccharides under heavy metal stress 
conditions indicate that these microorganisms are promising objects in the future. 

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Poster presentation 
250