Organizing co mmittee


). The structure of the synthesized substances was confirmed by IR,  1 H and  13 C NMR  spectra



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Abstracts ICPS 2023

6
).
The structure of the synthesized substances was confirmed by IR, 
1
H and 
13
C NMR 
spectra. 
 
 
 
 
 
N
CH
3
Cl
CHO
1
NH
NH
2
+
H
+
NaBH
4
CH
2
O
NH
NH
N
Cl
CH
3
NH
NH
N
Cl
CH
3
CH
2
NH
N
CH
2
N
Cl
CH
3
C
6
H
6
2
3
5
6
4
NH
N
N
Cl
CH
3
HC
A
B
reflux
H
+


Poster presentation 
251 
SYNTHESIS OF PHYTOHORMONES BY CYANOBACTERIA 
UNDER SALINITY CONDITIONS 
 
A. Usmonkulova, G. Kadirova, A. Abdullayev
 
Institute of Microbiology of the Academy of Sciences, Tashkent, Uzbekistan;
e-mail: usmonkulova.aziza@mail.ru 
It is known that most of the irrigated agricultural lands are losing their productivity due to 
soil salinity. This situation, in turn, has a negative impact on the amount of food products 
grown in agriculture. Therefore, to alleviate this, it is necessary to use natural biopreparations 
created on the basis of microorganisms free from chemical synthesis compounds and 
products. Cyanobacteria are oxygen-evolving photoautotrophs with worldwide distribution in 
every possible habitat, and they account for half of the global primary productivity. Because 
of their ability to thrive in a hostile environment, cyanobacteria are categorized as 
“extremophiles” [1]. Cyanobacteria synthesize a range of bioactive compounds that show 
plant growth promoting potential, and nowadays, cyanobacterial inoculants have been 
frequently utilized as a biofertilizers in the agricultural fields [2]. 
 
In this study, the synthesis of phytohormones (auxin and gibberellin) of local 
cyanobacterial cultures was observed during 3–9–14 days at 300–500–800 mM NaCl 
concentrations. According to the results of the studies, auxin was produced in very 
modest amounts at 800 mM salinity, and the amounts produced by the 
N. calcicola 32
and 
A. variabilis 28
strains increased by 3–4 times on the ninth day of cultivation 
compared to other days. The amount of auxin produced of cyanobacterial strains was 
found to be 2 times higher in the control comparison to 300 mM NaCl concentration 
and to decrease with increasing. 
A. variabilis 28
strain in particular produced 30 mkg/ml 
of auxin under control conditions, 14 mkg/ml at 300 mM NaCl, and 8 mkg/ml at 500 
mM NaCl. At various NaCl concentrations, the gibberellin synthesis of selected local 
cyanobacterial strains was observed for 3–9–14 days. It was discovered that in three 
days of growth, the 
N. calcicola 32
and 
A. variabilis 28
strains released about three 
times as much gibberellin under salinity circumstances and in the control option as 
compared to other days. On the third and ninth days of cultivation at a concentration of 
300 mM NaCl, 
N. calcicola 32
strain and 
Anabaena variabilis 28
strain were found to 
synthesis gibberellin in amounts of 29 and 15 and 11 and 6 mkg/ml, respectively. 
Synthesis of phytohormones by cyanobacteria at different concentrations of salinity 
means that these microorganisms are promising objects for agriculture. biopreparations 
created on the basis of local strains of cyanobacteria, in particular Nostoc and Anabaena 
genera, synthesize useful substances for plants and increase soil fertility. 

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