Organizing co mmittee

SYNTHESIS OF POLYCOMPLEXES OF MICROELEMENTS 
WITH POLYPEPTIDES 
 
O.M. Namozov, G.A. Usmanova, X.A. Mirzaxidov,
M.M. Karimov, T.M. Babaev 
 
National University of Uzbekistan, Tashkent. 100174. University campus,
e
-mail: [e-mail]
 
The complex formation of synthetic polymers with metal ions is a modern way of 
delivering macro- and microelements to a living organism, which are necessary for the 
development, maintenance, and restoration of cells and tissues. In the present study, this 
technique is extended to polypeptides obtained by alkaline hydrolysis of the silkworm. 
Systematic studies of the polypeptides themselves (fibroin and sericin) by molecular 
physics methods have not yet been carried out. The study is aimed at studying the 
mechanism and features of the complex formation of these polypeptides with micro-
elements (zinc, calcium, magnesium ions) by modern physical methods for studying 
macromolecular compounds in order to correct the balance of microelements in a living 
organism, modeling the processes occurring in it. Comparison of the hydrodynamic and 
dynamo-optical characteristics of metal-polymer complexes with polypeptides of 
various compositions and molecular weights made it possible to estimate the degree of 
binding and conformation of macromolecules in aqueous solutions. 
After alkaline hydrolysis of the silkworm, the fiber was washed with distilled water 
several times until a neutral medium (pH 6.0-6.5), after which the fiber was placed in 
solutions (50 ml) of 0.1 N. calcium chloride, magnesium sulfate, zinc sulfate (CaCl
2
; 
MgSO
4
·7H
2
O; ZnSO
4
·7H
2
O). 4 were prepared from each solution of 12 solutions, and 
0.2 g of fibroin were placed in each solution. The sorption of metal ions was measured 
by the conductometric method over time every 15, 30, 60 and 90 min. 
The method of IR spectroscopy was used to study silk samples before hydrolysis, the 
resulting polypeptide after silkworm hydrolysis, polymer metal complexes obtained 
with calcium, magnesium and zinc. The study conditions were the same. The studies 
were carried out in the laboratory of physical research of the Institute of Bioorganic 
Chemistry of the Academy of Sciences of the Republic of Uzbekistan named after. A.S. 
Sadykov. 
The original approach implemented in this work is the use of natural polypeptides 
obtained by hydrolysis of the silkworm as a polymeric carrier of metal ions. The use of 
these polypeptides for the study of complexation predetermines both the novelty of the 
study and the possibility of practical use in medicine of metal-polymer complexes based 
on them.