Organizing co mmittee

ANTI-INFLAMMATORY ACTIVITY OF PHENOLIC 
COMPOUNDS FROM TRANSFORMED ROOTS OF ARTEMISIA 
ANNUA L. AND A. VULGARIS L. 
A. Bashylau
1
, I. Zhavoronok
2
, A. Basalai
2
, P. Shabunya
3
, S. Fatykhova
3 
1
Central Botanical Garden of the NAS of Belarus, Minsk, Republic of Belarus 
2
Institute of Physiology of the NAS of Belarus, Minsk, Republic of Belarus 
3
Institute of Bioorganic Chemistry of the NAS of Belarus, Minsk, Republic of Belarus 
Ultrasonic extraction at elevated temperature can be used to increase the efficiency 
of extracting biologically active compounds from plant materials. Artemisia annua L. 
and Artemisia vulgaris L. are known for their medicinal properties, which are 
associated with their composition. One of the promising ways to obtain pharmaceutical 
raw materials is the biotechnology of "hairy" roots, however, the substances obtained 
from transformed plants should be examined for the composition and activity of 
metabolites. 
The objective of the work was to evaluate the composition of phenolic compounds in 
the extracts of the transformed roots of Artemisia annua and A. vulgaris, obtained using 
ultrasonic treatment, and to identify the anti-inflammatory effect of the extracts in the 
experiment. 
Materials and methods. The total content of phenolic compounds was determined 
according to the Folin-Ciocalteu method, the content of extractive substances was 
determined according to the State Pharmacopoeia of the Republic of Belarus. For HPLC 
analysis, the Agilent 1200 chromatograph with a diode array detector and the Agilent 
6410 mass selective detector was used. The study of anti-inflammatory activity was 
performed on male Wistar rats. Escherichia coli lipopolysaccharide was used to 
simulate a febrile response in the experiment. 
Results. With the help of sonication, extracts of transformed roots of Artemisia 
annua and A. vulgaris were obtained. The composition and content of phenolic 
compounds were determined, the presence of caffeylquinic acids and caffeic acid 
derivatives in extracts of two species of wormwood was established. Tests were carried 
out on laboratory animals with an experimental systemic inflammatory process. 
Conclusion. Ultrasonic extraction of transformed roots of Artemisia annua and A. 
vulgaris was effective in extracting phenolic compounds. In the obtained extracts, 8 
compounds were identified, mainly caffeylquinic acids and caffeic acid derivatives were 
identified. 
The study of the effect of extracts on deep body temperature in rats in normal 
conditions and with systemic inflammation led to the conclusion that the biologically 
active substances of wormwood have a modulating effect on thermoregulatory 
reactions. Intragastric administration of an extract of transformed roots of Artemisia 
annua 40 min before the systemic administration of E. coli LPS caused changes in the 
febrile response pattern, namely, it weakened the severity of phase II fever. 
The work was carried out within the framework of the international project N 
B21UKRG-005 (project manager H. Shutava). 
The authors are grateful to N. A. Matveeva, Doctor of Biological Sciences, for 
providing the culture of transformed roots for research.

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Poster presentation 
39