Organizing co mmittee

part which bound to the sorbent in the column and was detected between 10 and 20 
minutes. In the case of fractions, the HPLC chromatogram revealed that the intensity of 
the hydrophilic part decreases and the intensity of the hydrophobic part increases with 
increasing molecular weights of the fractions and vice versa. 
It can be concluded that potential anti-HIV, anticancer and antiradical peptides from 
the Cobra venom may be discovered. Low molecular peptides are less hydrophobic 
compared to high molecular peptides and proteins in the case of this object. Therefore, it 
could be speculated that new therapeutic agents with good water solubility may be 
developed on the base of low molecular peptides from 
Naja naja oxiana
. 
Isolation, identification, and deeper biological activity study of individual peptides, 
as well as investigation of their mechanism of action are planned for the near future. 

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Oral presentation
35 
BACTERICIDAL ACTION OF GENTIANA OLIVERI 
 
F. Ashurova, N. Mirakhmedova, M. Zalyalieva 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of the 
Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
[e-mail]
, 

The study of the properties of the biomedical activity of different plant species due to 
their less aggressive action and the absence of side effects is relevant. Particular 
importance is attached to the antimicrobial effects of plants on different types of 
microorganisms. Gentiana (Gentiana) is a fairly common plant, more than 400 species 
has medicinal value, mainly the root part is used. In Uzbekistan, Gentiana Oliveri is 
found, which is used in folk medicine for gastrointestinal disorders of various etiologies 
in the form of an infusion of dried aerial parts of plants. Purposeful study of the 
antimicrobial activity of extracts from the aerial part of Gentiana oliveri is an urgent 
task. 
The purpose of the study. To study the bactericidal activity in vitro of aqueous and 
alcoholic extracts of the aerial part of Gentiana oliveri. 
Material and methods. Aqueous, 30% and 40% alcoholic extracts were prepared 
from crushed aerial parts by continuous extraction. The antimicrobial activity of the 
extracts was studied on clinical cultures of bacteria isolated from patients undergoing 
treatment in RCH 1. 
Results and discussion. An aqueous extract of Gentiana oliveri inhibited the growth 
of Stafilococcus aureus by 67% higher than the control, gram-negative rods (
E. coli
, 
klebsiella) - by 16%. A 30% alcohol extract showed less bactericidal activity - 33% 
higher against St. aureus and 16% against E. coli and klebsiella. A 40% alcohol solution 
showed low bactericidal activity against Gram-positive St. aureus, and against gram-
negative bacteria from 16% to 33% relative to the control. Our results showed a high 
bactericidal activity of the aqueous extract of Gentiana oliveri against gram-positive 
microbes St. aureus. These data are important, since staphylococci are one of the most 
common microorganisms that cause purulent-inflammatory diseases: various tissues of 
the human body. 
Thus, our studies revealed a significant bactericidal activity of the aqueous extract of 
Gentiana oliveri. These data require further immuno-physiological study of the action of 
the aerial part of this plant, the mechanisms of its bactericidal activity. 

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Oral presentation 
36