Organizing co mmittee

 
 

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Poster presentation 
68 
CHEMICAL RESEARCH OF THE GENUS MYRICARIA (I) 
 
1
Ma Huan,
1
A.K. Umbetova, 
1
Janar Jenis, 
2
Sh. Sh. Sagdullaev, 
3
N.G. 
Gemedzhieva 
 
1
Al-Farabi Kazakh National University
 
Almaty, Kazakhstan. 
2
 S. Yu Yunusov Institute of the chemistry of plant substances of the Academy of 
Sciences of the Republic of Uzbekistan,
 
Tashkent, Uzbekistan.
 
3
Institute of Botany and Phytointroduction of the Ministry of Education and Science
 
Almaty, Kazakhstan. 
[e-mail] 
 
The flora of Kazakhstan is rich in promising little-studied plants that are used in 
traditional medicine, but additional research is required for their introduction into 
medicine. The objects of the study are the aerial part of the plant of the genus 
Myricaria 
(Myricaria bracteata Royle)
, collected in the Almaty region Enbekshikazakh district, 
the Zaili Altau ridge, the gorge of the Turgen River, in the vicinity of the village of 
Batan.
In this work, methods of investigating the quality of raw materials of the 
M.bracteata
plant have been worked out: humidity - 4.75%, total ash - 5.78%, sulfate ash - 10.85%, 
extractive substances - 25.54% (70% water-ethyl alcohol). The content of macro- and 
microelements was determined by atomic absorption spectroscopy. The highest content 
of iron is - 3.38% and potassium is - 0.35%.
The qualitative and quantitative composition of the plant was studied, carbohydrates, 
organic acids, coumarins, tannins, flavonoids and terpenoids were found. The highest 
content of tannins is 7.84%, carbohydrates - 6.77% and flavonoids - 4.12%. 
Technological parameters for obtaining the substance from the 
M.bracteata
plant have 
been worked out: the extractant - 70% water-ethyl alcohol, the ratio of the extractant 
and raw materials - 1:9, the extraction time - 24 hours, the temperature - 24-25℃. Fatty 
acid components were determined qualitatively and quantitatively by gas-liquid 
chromatography (GLC). During which 8 fatty acids were identified, among which 
unsaturated fatty acids predominate - linoleic - 47.8% and oleic acids - 33.4%, and 
among saturated ones - palmitic acid - 10.6%. Vitamins A, E were determined by the 
fluorimetric method of analysis, which showed that in the studied plant species the 
highest content of vitamin E (tocopherol) is 3.5 mg/100 g. In the aqueous extract, 20 
amino acids were identified by GLC methods, and vitamin C was determined by the 
titrimetric method in an amount of 10.1 mg/100 g. CO
2
extract was obtained by 
supercritical fluid extraction. The GC/MS method was used to study the component 
composition of the CO
2
extract, which identified 51 components in the carbon dioxide 
extract. The analysis of antioxidant components showed that the content of fat-soluble 
antioxidant was 1.67±0.03 mg/100g, and the content of water-soluble antioxidant was 
2.93±0.04 mg/100g.