Organizing co mmittee

 
 
 

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Poster presentation 
69 
INVESTIGATION OF THE CYTOTOXIC ACTIVITY OF 
COMPOUNDS OF ISOQUINOLINE ALKALOIDS AND THEIR 
SYNTHETIC DERIVATIVES 
E.O. Terenteva, U.B. Khamidova, M.R. Umarova, E.M. Lysova, V.I. Vinogradova,
Sh.N. Zhurakulov, A.A. Makhnyov, O.B. Alimukhamedova, L.K. Mustafina,
Sh.S. Azimova 
S. Yu. Yunusov Institute of the Chemistry of Plant Substences, M. Ulugbek Str. 77, 
100170, Tashkent, Uzbekistan; e-mail: 
[e-mail]
 
The cytotoxic activity of 48 isoquinoline alkaloids and their synthetic derivatives has 
been examined. The relationship between structure and function is established. It has 
been shown that cytotoxic activity depends on the presence of halogen derivatives in the 
molecule and on the length of the methylene chain. The effect of the most active 
compounds on the expression of the apoptosis genes Bcl2, Bax, and Casp3 was studied 
by PCR analysis. It was found that the most active compounds do not affect the 
expression of Bcl2, Bax, and Casp3 apoptosis genes, but reduce the expression of the 
JAK2 gene with the V617F mutation (a marker of chronic Ph-negative 
myeloproliferative diseases). 
 
 

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Poster presentation 
70 
BIODEGRADATION OF POLYETHYLENE IN THE EFFECT OF 
TEMPERATURE BY MICROORGANISMS 
 
I.M. Khalilov, M.M. Nazirov, F.B. Kobilov, N.A. Sherbekova 
 
Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, 7b 
Abdulla Qadiri Street, Tashkent 100128 
 
Plastic polymers are widely used in agriculture, industry and our daily life due to 
their convenience and low cost. At the same time, pollution with plastic polymers, 
especially polyethylene (PE), has a negative impact on the environment, as well as on 
the health of animals and people, since they are almost non-degradable in natural 
conditions. Biodegradation of PE in nature mainly occurs due to the biological activity 
of microorganisms after thermal oxidation. PE breaks down into low molecular weight 
substances such as alkanes, alkenes, ketones, aldehydes, various alcohols and fatty acids. 
Bacterial species such as fluorescens and 
P.aeruginosa
isolated from aqueous media 
have been shown to be capable of degrading polyethylene at a pH of 7 and a temperature 
of 30-37 
o
C. 
The purpose of the study is to study the effect of different temperatures on PE 
decomposition of bacterial strains belonging to the genus 
Pseudomonas
. 
Four strains of bacteria belonging to the genus 
Pseudomonas
were grown at 
temperatures of 35
o
C, 40
o
C and 45
o
C, and their effect on the degradation of PE was 
studied for 1 month. It was observed that the bacterial strains cultured in LPEM liquid 
medium had a reduced growth compared to the control medium. 40
o
C was found to be 
optimal for 
Pseudomonas
strains. Among the used strains, it was found that 
P.putida
and 
P.fluorescens
strains produced 24.21 and 16.36 times more organic compounds in 
the nutrient medium than the control one, respectively. In particular, the presence of 
these substances is shown in the absorption line between 190-200 nm (carbonyl groups) 
and 240-300 nm (aldehyde groups) of the UV spectrum. This indicates the formation of 
carbonyl groups and aldehydes in the food environment. It can be observed that the 
bacterial strains grown under the influence of the temperature of 45
o
C produced less 
organic compounds than at 40
o
C. In this case, we can see that 
P.stutzeri
, 
P.fluorescens
, 
P.putida
and 
P.aeruginosa
produced 17.51, 16.6, 22.25 and 13.47 times more organic 
compounds in the form of carboxyl and aldehydes compared to the control, 
respectively. It is important to note that 
Pseudomonas
strains grown at 35
o
C produced 
less organic compounds than those at 40
o
C and 45
o
C. 
P.stutzeri, P.fluorescens, P.putida 
and 
P.aeruginosa
bacterial strains at 35
o
C temperature are 10.89 compared to the 
corresponding control; 13.46; It was studied that 19.72 and 12.04 times more organic 
compounds were formed in the food environment. 
Thus, strains of bacteria belonging to the genus 
Pseudomonas
were observed to decom-
pose PE at different temperatures. It was observed that the growth and development range of 
Pseudomonas
strains was from 30 °C to 44 °C, and these bacterial strains degraded PE and 
produced organic matter even at these temperatures. Among the different temperatures 
used in the experiment, it was found that 
Pseudomonas
strains grown at 40
o
C had the 
highest PE degradation. It should be said that among the 
Pseudomonas
strains, 
P.putida
strain produced the largest amount of organic compounds (carboxyl and aldehydes) in the 
nutrient medium compared to 
P.stutzeri, P.fluorescens
, and 
P.aeruginosa
strains at all 
studied temperatures, and was considered an active PE-degrading strain. found. 

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Poster presentation 
71