Organizing co mmittee

CHEMICAL COMPONENTS OF Melilotus officinalis AND
Melilotus albus 
 
N.K. Usmanova
1
, A. R. Khurramov
2
,
Kh. M. Bobakulov
2
, E. Kh. Botirov
2 
 
1) 
Namangan State University, 
Namangan, Uzbekistan,
 
e-mail: [e-mail]
 
2) 
S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
 
Melilotus officinalis
(L.) Pall. - a biennial herbaceous plant of the 
Fabaceae
family. 
In the
folk medicine of Central Asia, preparations of melilot are used as an expectorant, 
emollient, analgesic, distraction, and sleeping aid, as well as for the
treatment of septic 
wounds. 
M. officinalis
has been found to have antioxidant, antibacterial, antitumor, and 
anti-inflammatory activity; the extract is active against the flu virus [1].
Melilotus albus
is a one- and two-year herbaceous plant with anticoagulative and 
fibrinolytic activity. 
M. albus
is used in folk medicine for thrombosis, infusion or 
decoction of the above-ground part of the plant as a lactogenic, anti-fever, for ascites, 
and for headaches.
Kempferol 
3-
O
-β-
D
-galactopyranoside, 
quercetin 
7-
O
-α-
L
-rhamnopyranoside, 
dihydrocoumarin, and melilotoside were isolated from various fractions of a 75% 
ethanol extract of M. officinalis above ground parts by column chromatography on 
silica gel and Sephadex LH-20, except for previously found compounds [2,3]. The 
isolated substances were identified based on UV, 
1
H, 
13
C NMR spectra, hydrolysis 
results, and comparison of physicochemical properties with the literature data. 
To
search for biologically active substances
,
we studied the component composition 
of the essential oil of the air-dried above-ground part of 
M. albus
growing in Uzbekistan 
and collected
it during flowering. 
The extraction of essential oil from crushed air-dry 
above-ground parts was carried out by hydrodistillation under atmospheric pressure. 
The
Qualitative and quantitative composition of essential oil was determined on an 
Agilent 5975C Inert MSD/7890A GC chromatography-mass spectrometer (Agilent 
Technologies, USA). By the
GC-MS method, 5 compounds were identified in the 
composition of essential oil from the air-dry plant, which is 98.93% of the total amount 
of essential oil. The main component of the essential oil is coumarin, which content is 
97.92%. Benzyl alcohol (0.30%), 2-hexenal (0.27%), dihydrocoumarin (0.26%), and 
phenyloxyran (0.08%) were found in the essential oil, except coumarin.
As a result of pharmacological studies, it was found that extracts and individual 
compounds isolated from 
M. officinаlis
possess antioxidant, anticoagulant, and anti-
diabetic properties. Quercetin 7-
O
-α-
L
-rhamnopyranoside has antiviral activity.