Organizing co mmittee

CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY 
OF Perovskia botschantzevii
ESSENTIAL OIL 
O.K. Askarova
1
, Kh.M. Bobakulov
2
, A.M. Yarylkaganova
2
, S.A. Sasmakov
2
,
E.Kh. Botirov
2
, Sh.S. Azimova
3
 
1) Namangan Institute of Engineering and Technology, Namangan, Uzbekistan
2) S.Yu.Yunusov Institute of the Chemistry of Plant Substance, Academy of Science of 
the Republic of Uzbekistan, 
e-mail: [e-mail]
 
Perovskia botschantzevii 
Kovalevsk. & Koczk
(Lamiacaeae):
semi-shrub found in 
Kyrgyzstan, Uzbekistan, Tajikistan, and Afghanistan on rocky slopes, pebbles, and river 
valleys. The chemical composition of 
P. botschantzevii
is practically unstudied [1]. 
In order to search for biologically active compounds, we studied the composition of 
the essential oil of the aerial part of 
P. botschantzevii
, collected during the flowering 
period. The extraction of essential oil from crushed fresh and air-dry aerial parts was 
carried out by hydrodistillation at atmospheric pressure for 2.5 hours. 
According to GC-MS data, the main components of the essential oil of both the air-
dry and fresh aerial parts are 1,8-cineol; its content is 34.8 and 40.7%, respectively. 
Bornyl acetate (12.3%), alloaromadendren (5.5%), borneol (5.4%), Δ-3-karene (5.3%), 
β-caryophyllene (5.2%), o-cymene (4.9%), camphor (4.1%) and other compounds. In 
addition to 1,8-cineol, the dominant components of fresh plant essential oil are Δ-3-
karene (8.6%), borneol (7.7%), bornyl acetate (7.0%), alloaromadendren (3.6%), and β -
-caryophyllene (3.2%).
1,8-Cineol (eucalyptol) belongs to the bicyclic monoterpenes and is used for 
respiratory diseases such as bronchitis, the common cold of the respiratory tract, chronic 
and inflammatory respiratory diseases, asthma, and hay fever. The aerial part of 
P. 
botschanzevii
can serve as a rich source of 1,8-cineole. 
A modified agar diffusion method was used to study the antibacterial, and antifungal 
properties of 
P. botschanzevii
essential oil. Microorganism strains 
Bacillus subtilis, 
Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli,
and one 
Candida 
albicans
fungal strain were used as test cultures. The results of 
in vitro
antimicrobial 
tests showed that all the studied microorganisms were sensitive to the action of the 
essential oil from 
P. botschanzevii. 
At the same time, the greatest antibacterial effect 
was observed in relation to the gram-positive strain of bacteria 
Bacillus subtilis.
This 
activity of the essential oil may be due to the presence of 1,8-cineol in its composition, 
while the antimicrobial activity of the studied sample may vary significantly depending 
on the chemotaxonomic characteristics of the plant as well as the biological properties 
of the test microorganisms used.