Organizing co mmittee

Concentrations of non-essential amino acids (mg/g)
Asp 
Glu 
Ser 
Gly 
Asn 
Gln 
Cys 
Ala 
Pro 
Tyr 
Total 
1
*
 
0.24 
- 
0.13 
0.03 
0.65 
1.04 
0.44 
0.06 
0.3 
0.2 
3.09 
2
* 
0.08 
- 
0.08 
0.02 
0.43 
0.34 
0.53 
- 
0.38 
0.11 
1.97 
3
* 
0.91 
0.38 
0.59 
3 
3.72 
1.05 
1.92 
- 
0.46 
1.1 
13.13 
4
* 
0.07 
- 
012 
0.63 
0.79 
0.52 
0.54 
- 
0.09 
0.11 
2.87 
Concentrations of essential amino acids (mg/g)
Thr
Arg 
Val 
Met 
Ile 
Leu 
His 
Trp 
Fln 
Lys 
Total 
1
* 
0.46 
0.11 
0.,27 
1.34 
0.17 
0.38 
0.08 
0.18 
0.1 
0.76 
 3.85 
2
* 
0.34 
0.05 
0.09 
1.02 
0.18 
0.41 
0.06 
0.07 
0.02 
0.95 
 3.19 
3
* 
0.88 
0.98 
0.71 
1.2 
0.41 
0.94 
0.35 
1.38 
1.04 
1.35 
 9.24 
4
* 
0.46 
0.31 
0.41 
0.87 
0.2 
0.44 
0.08 
0.09 
0.06 
0.85 
 3.77 
Note:
1
*
- 
C. spinosa leaves
(Southern Aralkum)
 
2
*
-
C. spinosa roots
(Southern Aralkum), 
3
*
-
C. spinosa leaves
(Samarkand region),
 
4
*
-
 C. spinosa 
roots (Samarkand region) 
The biosynthesis of the sulfur-containing amino acids was demonstrated to increase as 
a response to abiotic stress in the plants. Concentrations of cysteine (0.44-0.53mg/g) involving to 
the synthesis of glutathione indispensable for the elimination of the oxidative stress caused by the 
abiotic one were found significantly reduced as compared to those in the tolerant species (1.92-
0.53mg/g); no traces of glutamine were found. Methionine is involved in the initial stages of 
biosynthesis of aliphatic glucosinolates; our findings demonstrate that concentrations of 
methionine in the plants growing in the Southern Aralkum, that is in the conditions of high soil 
salinity, were increased as a response to the stress (1.02-1.34 mg/g), while the concentrations in 
question were found unchanged in the salt-tolerant species (0.87-1.2 mg/g).

---

Poster presentation 
139 
VITAMINS FOUND IN LEAVES AND FRUITS OF Lycium 
ruthenicum GROWING IN THE SOUTHERN ARAL SEA REGION
 
S.Sh. Abdirahimova, Sh.S. Olimjonov, J.F. Ziavitdinov 
A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, 
e-mail: [e-mail]  
Plants belonging to the 
Solanaceae
family are known to contain peptides, proteins, 
alkaloids, glycosides, vitamins and many other bioactive substances. Worldwide, 
attention of many researchers is attracted to the manifestations of hormonal, 
antimicrobial, antiviral and anticancer activities that can be seen as typical of the 
compounds.
The work was initiated to identify vitamins containing in the samples of various 
organs of 
Lycium ruthenicum
Murr., belonging to the 
Solanaceae
family, and to 
determine their concentrations. 
To determine concentrations of the B group vitamins, including B1, B2, B3, B6, B9 
as well as those of K and C, the crushed powders of plant organs were extracted by the 
distilled water to be centrifuged at 6,000 rpm. The supernatants were selected; the 
proteins and peptides were precipitated by means of 10%
 
trichloroacetic acid. To 
separate the sediments, the solutions were centrifuged at 6,000 rpm. The supernatants 
were neutralized by 0.1M NaOH adding to the necessary volume. High performance 
liquid chromatography was used to determine concentrations of vitamins in the 
neutralized extracts.
The extracts obtained from the leaves and fruits
 
of the plant under study were 
analyzed. Presence of vitamins in each sample was identified by comparing the elution 
times of experimental samples to those of the standard column-analyzed vitamins; the 
concentrations were calculated using the trend equations by fitting the appropriate peak 
areas for vitamins in the chromatograms. The findings can be seen in the Table.
Table. Concentrations of water-soluble vitamins in the samples of the 
Lyceium 
ruthenicum 
leaves and fruits (μg/g, М±n, n=3) 
Plants 
С 
РР 
В1 
В6 
В9 
В2 
Concentrations of water-soluble vitamins, μg/g 
Lycium ruthenicum
leaves
 
- 
- 
0.184 
1.803 
12.691 
0.389 
Lycium ruthenicum 
fruits
 
0.309 
- 
- 
1.747 
10.442 
0.419 
Samples of the 
Lycium ruthenicum
leaves and fruits were analyzed for presence and 
concentrations of water-soluble vitamins, to name ascorbic acid and PP, as well as the B 
group vitamins, including B1, B2, B6 and B9. The leaves and fruits of the plant were 
found not to contain any concentrations of PP; ascorbic acid was found in the fruits 
only. Concentrations of B9 were found significantly higher than those of other vitamins 
under study.