Phylogenetic Analyses
The Maximum Parsimony (MP), Maximum Likelihood (ML), NJ and
Bayesian analyses were performed using PAUP* 4.0b10 [42].
Maximum Parsimony (MP) Analysis
Maximum Parsimony (MP) analysis was performed with the follow-
ing settings: heuristic search algorithms with tree bisection recon-
necting (TBR) branch swapping, MULPARS in effect, all characters
equally weighted, gap treated as missing characters, zero length
braches collapsed, random addition sequence set to 1000 repli-
cates, and branch swapping limited to 10000000 rearrangements
per replicate. When maximum parsimonious tree were saved, a
strict consensus tree [43] was constructed. Bootstrap analysis [44]
was performed using 1000 replicates, with the random addition
sequence set to 10, and branch swapping limited to 10000000 rear-
rangements per replicates.
Table 4- ITS sequences of nrDNA retrieved from GenBank for phy-
logenetic analyses
Table 5- Generic classification of Limonium
International Journal of Molecular Biology
ISSN: 0976-0482 & E-ISSN: 0976-0490, Volume 6, Issue 1, 2015
Al-Ghanem S.M.S.
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