Materials and methods
Animals
The experimental were carried out on white male Wistar rats (body mass of 200-220 g, 6-8 weeks of age) that were maintained in the vivarium of the Institute of Cell Biophysics, Russian Academy of Sciences (ICB RAS), Pushchino. Investigations on laboratory animals were conducted in accordance with the guidelines of the "European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes" and the legislation of the Russian Federation.
The animals had access to food and water ad libitum. Their feeding was discontinued at 24 hours before surgery.
Recombinant human Prx VI was obtained in the Laboratory of Reception Mechanisms in ICB RAS, Pushchino, Prx VI specific activities being as follows: the peroxidase sp.activity being 80 nmol/mg/min H2O2, and 60 nmol/mg/min tert-butyl hydroperoxide.
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