Glucose
GOD-POD.
Liquid
MDBSIS46-I 03/05/17
SPINREACT,S.A/S.A.U
Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99. e-mail:
spinreact@spinreact.com
GLUCOSE -LQ
Quantitative determination of glucose
IVD
Store at 2-8ºC
PRINCIPLE OF THE METHOD
Glucose oxidase (GOD) catalyses the oxidation of glucose to gluconic
acid. The formed hydrogen peroxide (H
2
O
2
),
is detected by a
chromogenic
oxygen acceptor, phenol, 4
– aminophenazone (4-AP)
in the presence of peroxidase (POD):
-D-Glucose + O
2
+ H
2
O
GOD
Gluconic acid + H
2
O
2
H
2
O
2
+ Phenol + 4-AP
POD
Quinone + H
2
O
The intensity of the color formed is
proportional to the glucose
concentration in the sample
1,2
.
CLINICAL SIGNIFICANCE
Glucose is a major source of energy for most cells of the body; insulin
facilitates glucose entry into the cells.
Diabetes is a disease
manifested by hyperglycemia;
patients with
diabetes demonstrate an inability to produce insulin
1,5,6
.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
REAGENTS
R
TRIS pH 7.4
Phenol
Glucose oxidase (GOD)
Peroxidase (POD)
4
– Aminophenazone (4-AP)
92 mmol/L
0,3 mmol/L
15000 U/L
1000 U/L
2,6 mmol/L
