REFERENCE VALUES 1 Serum or plasma:
60
– 110 mg/dL
3,33
– 6,10 mmol/L
These values are for orientation purpose; each laboratory should establish
its own reference range.
PERFORMANCE CHARACTERISTICS Measuring range: From
detection limit 0,3709 mg/dL to
linearity limit 500
mg/dL.
If the concentration is greater than linearity limit dilute 1/2 the sample with
ClNa 9 g/L and multiply the result by 2.
Precision: Intra-assay (n=20)
Inter-assay (n=20)
Mean (mg/dL)
98,5
264,6
92,5
250
SD
0,5754
1,2733
2,76
6,44
CV (%)
0,59
0,48
2,98
2,57
Sensitivity: 1 mg/dL = 0,0039 (A).
Accuracy: Results obtained using SPINREACT reagents (y) did not show
systematic differences when compared with other commercial reagent (x).
The results obtained using 50 samples were the following:
Correlation coefficient (r)
2
: 0,99492.
Regression equation: y=1,104x - 1,249.
The results of the performance characteristics depend on the analyzer used.
NOTES 1.
Calibration with the aqueous standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a
serum Calibrator.
2.
Use clean disposable pipette tips for its dispensation.
BIBLIOGRAPHY 1.
Kaplan L.A. Glucose. Kaplan A et al. Clin Chem The C.V. Mosby Co. St
Louis. Toronto. Princeton 1984; 1032-1036.
2.
Trinder P. Ann Clin Biochem 1969; 6 24-33.
3.
Young DS. Effects of drugs on Clinical Lab. Tests, 4th ed AACC Press,
1995.
4.
Young DS. Effects of disease on Clinical Lab. Tests, 4th ed AACC 2001.
5.
Burtis A et al. Tietz Textbook of Clinical Chemistry, 3rd ed AACC 1999.
6.
Tietz N W et al. Clinical Guide to Laboratory Tests, 3rd ed AACC 1995.
