L. A. Kadirova, A. A. No’monjonov bioinformatika o‘zbekiston Respublikasi vazirligi tomonidan ta’lim yo‘nalishi bo‘yicha darslik sifatida tavsiya etilgan

GenBank -dan H5N1 ketma-ketliklaridan birini oling va keyin o'xshash ketma-ketliklarni topish uchun BLAST-dan foydalaning . Topilgan ketma-ketliklarning xususiyatlari va lokalizatsiyasi qanday?
b ) noma'lum kelib chiqishi va noma'lum funktsiyalarning uchta ketma-ketligi berilgan:
- mRNK:
acauuugcuu cugacacaacuguguucacuagcaaccucaaakagacaccauggugcacc
ugacuccuga ggagaagucugcgguuuacugcccuguggggcaaggugaacguggaugaag
uugggguga ggccgggcaggcugcugguggucuacccuuggacccagagguucuuug
aguccuuugg ggaucuguccaccugaugcaguuaugggcaaccuaagg ugaaggcuc
aggcaagaa _
gcaccuuugc cacacugagugagcugcacugugacaagcugcacguggauccugagaacu
ucaggcuccu gggcaacgugcuggucugugugugcggcccaucacuuuggcaaagaauuca
ccccaccagu gcaggcugccuaucagaaagugguggcugguguggcuaaugcccuggcccc
akaaguauka _
cuaaguccaa cuacuaaaacugggggauuaugaagggccuugcaucuggauucugcc
uaauaaaaaa cauuuauuuu cauugc - cDNK:
acatttgctt ctgacacaac tgtgttcact agcaacctca aacagacacc atggtgcacc tgactcctga ggagaagtct gcggttactg ccctgtgggg caaggtgaac gtggatgaag ttggtggtga ggccctgggc aggctgctgg tggtctaccc ttggacccag aggttctttg agtcctttgg ggatctgtcc actcctgatg cagttatggg caaccctaag gtgaaggctc atggcaagaa agtgctcggt gcctttagtg atggcctggc tcacctggac aacctcaagg gcacctttgc cacactgagt gagctgcact gtgacaagct gcacgtggat cctgagaact tcaggctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaagaattca ccccaccagt gcaggctgcc tatcagaaag tggtggctgg tgtggctaat gccctggccc acaagtatca ctaagctcgc tttcttgctg tccaatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact gggggatatt atgaagggcc ttgagcatct ggattctgcc taataaaaaa catttatttt cattgc
Aminokislotalar ketma-ketligiga tarjima:
HCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGV ANALAHKYH
BLAST bilan qidirish uchun ushbu ketma-ketliklarning har biridan foydalaning . Qidiruv natijalarini baholang. Maydonlarning ma'nosi nima Bal , Baho va identifikatorlar (savolga javob berish uchun natijalar sahifasini > bilan boshlanadigan qatorlarga aylantirishingiz kerak)?

• 
  Bu ketma-ketliklar qaysi organizmlarga tegishli?
  
• 
  Ushbu genda kodlangan oqsil qanday vazifani bajaradi?
  
• 
  Qaysi ketma-ketliklar o'rganilayotgan DNK hududiga juda yaqin?
  

Ular qanday organizmlarga mansub va ular qanday vazifani bajaradi?

• 
  Uchta qidiruv so'rovi bir xil natijalarga erishdimi?
  
• 
  mRNK va cDNK ketma-ketligidan 21 ta nukleotidni va oqsillar ketma-ketligidan mos keladigan aminokislotalarni ehtiyotkorlik bilan olib tashlang. U BLAST qidiruv natijalarini o'zgartirdimi?
  
• 
  Ketma-ketlik oxirida ba'zi nukleotidlarni/aminokislotalarni ehtiyotkorlik bilan olib tashlang va yana BLAST qilishga harakat qiling.
  

Mavzu bo'yicha savollar.

1. 
   Xuddi shunday natijalarga erishdingizmi? Ballar va identifikatsiyalarning qiymatlari qanday ?
   
2. 
   Qidiruv oldingi natijalarni berishni to'xtatishi uchun qancha nukleotid/aminokislotalarni olib tashlash kerak?
   
3. 
   Agar siz nukleotidlarni/aminokislotalarni ketma-ketlikning o'rtasidan olib tashlasangiz nima bo'ladi?
   
4. 
   Agar ketma-ketlikning o'rtasidan 1\2\3 nukleotidni olib tashlasangiz, DNKning aminokislotalar ketma-ketligiga tarjimasi bilan nima sodir bo'ladi?