Organizing co mmittee

 
 
№ Sample name 
Content N,
%
Content,
% 
Degree of 
binding, % 
Productivity, 
% 
1 
CS initial 
8,21 
- 
- 
- 
2 
CS: СA 1:1 
4,54 
57,0 
55,0 
52,0 
2 
CS: СA 2:1 
5,71 
32,7 
71,7 
80,0 
4 
CS: СA 3:1 
6,30 
31,3 
96,0 
85,7 
5 
CS: СA 4:1 
7,12 
26,1 
96,2 
82,8 

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Poster presentation 
232 
UV-SPECTROSCOPIC STUDIES CHITOSAN SULFATE 
Bombyx mori 
 
V.N. Rakhmanova, M.A. Muslimova, N.S. Ashurov, S.S. Rashidova 
Institute of Chemistry and Physics of Polymers ASRU, 100128, Tashkent, 
A. Kadyri str. 7 b,
е-mail: [e-mail] 
 
It is known that chitosan does not dissolve in water and this to a certain extent limits 
its practical application. The production of water-soluble chitosan derivatives expands 
its range of applications in biomedicine. One of the ways of obtaining water-soluble 
chitosan derivatives is its interaction with acids of different nature.
 
In recent years, the 
interest in these objects sharply increased thanks to numerous studies, which showed 
that when switching from micro- to nano-particles there occurs qualitative change in 
many physical and chemical properties of substances, the nature of kinetics of chemical 
processes and particles flowing on the surface.
In this work, the nanoparticles of sulfochitosan were obtained and their structural 
properties were investigated via UV-Spectroscopy. 
The results obtained via UV-Spectroscopy show that with the increase of substitution 
degree in chitosan sulfate a noticeable change of absorption band happens within the 
range of 200-220 nm and 270-280 nm which may be related with n-

* and n-

* 
transitions of nitrogen electrons and oxygen of the original chitosan as well as 
substituted sulfogroups S=O. 
Changes in absorption bands 
may be related with the formation 
of covalent links between chitosan 
amino groups and chlorosulfonic 
acid, which leads to a decrease in 
electronic transitions n-

* of an 
unshared pair of nitrogen electrons.
It should be noted that with a 
component ratio of 1:10 the low 
molecular 
weight 
fractions 
of 
chitosan sulfate were formed as a 
result of a strong destruction of 
macromolecules. Decrease in band 
intensity within the range of 270-
280 nm was detected. 
Thus, the research results suggest that the most optimal conditions for the production 
of chitosan sulfate Bombyx mori with a high degree of substitution and low destruction 
are duration 3 hours, chlorosulfonic acid:chitosan ratio of 6:1 and the temperature of 
50
o
C. It was established that when stored during 1 month, the samples of chitosan 
sulfate solutions with varying degrees of substitution will stay stable.