Organizing co mmittee

 
 
№ 
Samples isolated from 
plants 
Critical concentration 
of micelle formation 
(kg m
-3
) 
Surface tension 
σ(mN/m
-1
) 
1. 
Rumex confertus 
3,51 
30 
2. 
Rumex pomiricus 
2,24 
35 
3. 
Rumex syriacus 
4,39 
28 
4. 
Rumex aquaticus 
4,56 
35 
5. 
Rumex tianschanicus 
4,13 
57 

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Poster presentation 
142 
ANTIBACTERIAL AND ANTIMICROBIAL
ACTIVITY OF 
EXTRACTS AND FRACTIONS OF MEDICINAL PLANTS
Rumex halaezii, Rumex syriacus 
 
N.M. Ashirmatova
1
, K.A. Eshbakova
1
, B.D. Mamarasulov
2 
 
1) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
e-mail: 
[e-mail]
 
2)
Institute of Microbiology of the Academy of Sciences of Uzbekistan, Tashkent, 
Republic of Uzbekistan 
 
Plants belonging to the genus 
Rumex
make up the 
Polygonaceae
. family with more 
than 200 species. It is distributed mainly in the northern temperate regions. Mostly 
perennial herbs with strong taproots, lanceolate inflorescences and enlarged spiral-
triangular fruits. The name "
Rumex
" comes from the Greek word for "spear" and refers 
to the shape of its leaves. Varieties belonging to the 
Rumex
species occupy a valuable 
placein the world. 
In addition, the antimicrobial fraction and activity of 
R. holaezii 
and
R. syriacus
leaves and roots extracts against test strains were determined (Table 1). 
Antibacterial activity of R. 
holaezii
and 
R. syriacus
roots extracts and leaves extracts 
by well diffusion method.
Table 1
 
Pathogen
test strains
R. holaezii
 
R. syriacus
 
Positive
control
canamycin 
Negatie 
control
(DMSO)
roots 
extract 
70% 
ethanol
leaf extract 
70% 
ethanol
root 
extract
70% 
ethanol
leaf extract 
70% 
ethanol
Diffusion 
mm
E. coli 
0 ± 00 
0 ± 00 
0 ± 00 
0 ± 00 
21 ± 0.13 
0 ± 00 
B. subtilis 
6 ±1.02 
6 ± 0.13 
6 ± 1.21 
8 ± 0.16 
24 ± 1.43
4 ± 0.32
P.aureginosa 
8 ± 00 
6 ±1.10 
10 ± 00 
12 ± 00 
26 ± 1.47 
5 ± 0.36 
S. aureus 
6 ± 1.42 
11 ±1.32 
16 ± 0.14 19 ± 00 
22 ± 1.38 
4 ± 0.27 
C. albicans 
14 ± 0.7
8 
± 0.21
12 
± 
0.11
13 
± 0.04
21 
± 1.36
6 
± 0.14
Figure 1: Antimicrobial activity of 
R. holaezii, R. syriacus 
plants in root and leaf 
extracts 

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Poster presentation 
143 
ISOLATION OF THE TOTAL PROTEINS OF Arundo donax ROOT 
AND THEIR ANTIMICROBIAL ACTIVITIES 
 
Kh.Kh. Dolimov
1
, F.B. Eshboev
1
, I.J. Jalolov
2
, M.M Mirzaolimov
2
, 
A.A Abdumamatov
2
, U.A. Dolimova
2
, Sh.S Azimova
1 
 
1) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent
2) Fergana State University
Arundo donax 
is perennial plant belonging to the 
Poaceae
family, 
Arundinoideae
subfamily. 
Arundo donax
is considered one of the medicinal plants, and according to 
tradition, rhizomes are used in the treatment of chest, dental diseases, cystitis, diuretic 
diseases, and a mixture of roots and honey is used in the treatment of cancer. The stem 
contains tannins, up to 48% sugar and lactic acid, up to 6% proteins and 3% fats, and 
the amount of alkaloids is 0.46% of the dry biomass of the plant. However, there is not 
enough information about the proteins isolated from the root of this plant and their 
biological activities. Therefore, the purpose of this research was to isolate common 
proteins from the root of the plant and study of their antimicrobial activity. 
During the research, total proteins were isolated from the root of 
Arundo donax
. For 
this, 100 g of the root of the plant was taken, crushed in a homogenizer and extracted 
using a 0.2 M solution of NaOH. Proteins in the obtained extract were precipitated 
using a saturated solution of ammonium sulfate and dialyzed to remove various ions 
from the composition. After dialysis, the amount of proteins was determined in Lowry's 
method and it was found that 100 g of root contains 1 g of protein. 
After that, the antimicrobial activity of the isolated proteins was evaluated using five 
species of microorganisms: Gram-positive bacteria 
Bacillus subtilis
(RKMUz - 5) and 
Staphylococcus aureus
(ATCC 25923); Gram-negative bacteria 
Escherichia coli
(RKMUz - 221) and 
Pseudomonas aeruginosa
(ATCC 27879); the fungus 
Candida 
albicans
(RKMUz - 247). According to the results of the antimicrobial activity, the 
proteins of 
Arundo donax
root did not show antimicrobial activities against selected 
microorganisms
.