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Poster presentation 
160 
1-O-METHYLEMODIN AND STIGMASTEROL COMPOUNDS 
FROM THE UNDERGROUND PART OF RUMEX PAMIRICUS
G.D. Shermatova
1
, K.A. Eshbakova
2
, Sh.Sh. Sagdullaev
2
, B.S. Mirzaev
1
 
 
1) “TIIAME” National Research University, 100000, Tashkent, 39, Kari-Niyaziy str.,
e-mail: [e-mail]
2) S.Yu. Yunusov Institute of the Chemistry of Plant Substances Academy of sciences of 
the Republic of Uzbekistan st. Mirzo-Ulugbek, 77, 100170 Tashkent 
The basis of the technology for the isolation of 1-
O
-Methylemodin and Stigmasterol 
(Fig.1) compounds is the extraction of raw materials with various organic solvents 
followed by chromatographic purification. Classical extraction methods (percolation 
and maceration) are time-consuming and laborious.
The herb 
Rumex pamiricus
Rech. f. belongs to the family of Polygonaceae and there 
are over 250 types on the earth and 16 types in Uzbekistan. 
The roots of the herb 
Rumex pamiricus
dried at room temperature, in shade. The 
pounded herb roots were first subjected to extraction in chloroform, then three times in 
70% acetone hydrous solution. The acetone extract was distilled under vacuum; the 
remaining water solution was subjected to extraction with ethyl acetate. Ethyl acetate 
extracts were collected and were dehydrated by adding anhydrous salt Na
2
SO
4
. The 
dehydrated extract was filtered, its concentration increased under vacuum, the total 
phenols were precipitated by adding pure hexane to the condensed extract. The created 
precipitate was washed, and filtered and the extracted total phenols of chloroform and 
ethyl acetate fractions constituted 3.4% of the herb dry weight. 
The chloroform fraction subjected with column chromatography on KSK silica gel, 
eluted with a mixture of extraction benzene–ethyl acetate: (50:1, 40:1, 30:1, 20:1 and 
10:1). The structure of 1-
O
-methylemodin and stigmasterol was established on the basis 
of the analysis of the data of MS (Mass spectrometry), 
1
Н and 
13
С NMR spectra 
(Nuclear Magnetic Resonance), and of the DEPT (Distortionless Enhancement of 
Polarization Transfer), HSQC (Heteronuclear Single Quantum Coherence) and HMBC 
(Heteronuclear Multiple Bond Correlation) experiments. Qualitative analyses of major 
phenolics by TLC (Thin Layer Chromatography) analysis were also evaluated.