Organizing co mmittee

partial agonists of A
1
type adenosine receptors. 
This work was supported by a grant from the Russian Science Foundation (Project 
No. 21-13-00429). 

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Oral presentation 
20 
ESSENTIAL OIL COMPOSITION AND BIOACTIVITY OF 
TRIPOLIUM PANNONICUM L. 
Raigul Suleimen
1
, Yerlan M. Suleimen
2,3,4*
, Zharkyn A. Ibatayev
5
, 
Meruyert Ashimbayeva
2,3
, Petr Gorovoy 
6
, Tokay Tursonjan
2
 
 
1
 L.N. Gumilyov Eurasian National University, Kazakhstan, Astana, K. Satpayev st, 2. 
2
 Nazarbayev University, Kazakhstan, Astana, Kabanbay Batyr ave, 53. 
3
Kazakh University of technology and business, Kazakhstan, Astana, Mukhamedkhanov, 
37A 
4
 Sh. Ualikhanov Kokshetau University, Kazakhstan, Kokshetau, Abaya st, 76 
5
S. Seifullin Qazaq Agrotechnical University, Astana, Kazakhstan 
6
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian 
Academy of Sciences, Vladivostok, Russia 
 
Tripolium pannonicum 
subsp. 
tripolium 
(L.) Greuter (
Syn.
 
Aster
tripolium
L.), a
perennial plant native 
to Europe, is a member of the Asteraceae Family, typically 
habitat of marine setting and owned purple daisy-like flowers, flowering from July to 
October. 
The 
T. pannonicum 
plant was collected in Far East Shkotovo District, near the 
Shkotovo Township, 1 km southwest of the village between the estuaries of the rivers 
Shkotovka (Tsimuhe) and Artemovka by Prof. P.G. Gorovoy. 
EOs of 
T. pannonicum 
was obtained from dried, crushed aerial parts by 
hydrodistillation in a Clevenger apparatus for 2 h according to the method described in 
Pharmacopoeia. The total yield percentage of EOs from dried aerial parts of 
T. 
pannonicum 
was 0,19%. 
The EOs composition was determined by a Clarus-SQ 8 GC (PerkinElmer) with a 
mass-spectrometric detector. The EOs composition was identified based on the 
comparison of their retention indices in relation to (C
9
-C
24
) n-alkanes with those of 
published data or with authentic standards. Also, the compounds were identified by 
comparing their MS data with those from the NIST mass spectral library and available 
mass spectra and, wherever promising, using co-injection of authentic standards. The 
percentage contents of the extract constituents were calculated by internal normalization 
of the constituent peak areas. In the EO of 
T. pannonicum 
was found more than 50 
compounds and the most abundant compounds (with % > 3.0) were 
α
-pinene (20.7%), 
β
-pinene (8.5%), myristicin (4.3%), limonene (4.1%), 
α
-myrcene (3.4%) and 
caryophyllene oxide (3.4%). 
In addition to the chemical composition, also larvicidal and free radical scavenging 
activities of the EO has been studied. Larvicidal activity was studied using 
A. salina 
larvae. EOs did not exhibit any toxicity on 
Artemia salina 
larva. The free radical 
scavenging activity was assessed by using DPPH radical scavenging assay. The EO 
showed weak DPPH radical scavenging activity. 
The present study highlighted EO of 
T. pannonicum 
from Far East Asia. The 
chemical composition of the EO of 
T. pannonicum 
from Far East Asia was studied for 
the first time. It was found that the EO of 
T. pannonicum 
has low antiradical activity 
and no lauricidal activity. 
Funding
: 
This research was funded by the Science Committee of the Ministry of 
High Education and Science of the Republic of Kazakhstan (Grant No AP13067774»). 

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Oral presentation
21