Organizing co mmittee

Key Words:
Thalictrum minus
L., medicinal plants, alkaloids, preparative thin layer 
chromatography and NMR spectroscopy. 
OBJECTIVES: Thalictrum minus
L, a plant of the genus 
Thalictrum
, is one of the 
medicinal plants that are widespread in the flora of Azerbaijan and contain alkaloids, 
flavonoids, triterpene glycosides, saponins, and other biologically active substances. 
The 
Th. minus
plant, which mainly contains isoquinoline and aporphine group 
alkaloids, has been used in folk medicine for anti-inflammatory, anti-infection, anti-
tumor, anti-malarial, and nervous system diseases. The sedative effect was found in 
experiments. It is known from world literature that alkaloids have a wide range of 
effects and cause poisoning when taken in large amounts. For this reason, our goal is 
to conduct a preliminary analysis of the alkaloids found in the root of the 
Th. minus
plant, which is common in Azerbaijan. 
METHODS:
The raw material for the study was the root of the 
Th. minus
plant 
collected in the Altiagach settlement in July 2020, crushed and dried in the shade. 
Crushed raw material (200 g) is concentrated by extracting 3 times with 75% 
methanol. The solid residue is basified with a 10% ammonia solution and extracted 
with chloroform and dichloromethane to give the alkaloid complex. The obtained 
aggregate is analyzed firstly by thin layer chromatography (TLC) method then by 
preparative TLC (PTLC) method (Chromatography plates: Merck (Germany); solvent 
system: methanol - dichloromethane - 25% ammonia solution (8:2:0.1), clarifier: 
Dragendorf reagent). NMR spectra of individually obtained alkaloids are recorded 
with a Bruker 600MHz Avance III spectrometer. 
RESULTS.
Alkaloids of the root of 
Th. minus
plants growing in Azerbaijan were 
studied. By PTLC method individual alkaloids were separated from this part. To 
determine the chemical structure of the separated alkaloid which has an R
f 
-0.51, 
identified by NMR spectrsocopic method.
CONCLUSION.
The alkaloid of the root of 
Th. minus
from Azerbaijan flora, were 
investigated and it was determined as berberine. 

---

Poster presentation 
62