Organizing co mmittee
Yüklə 5,12 Mb. Pdf görüntüsü
|
- Bu səhifədəki naviqasiya:
- , D.M. Amonova 1 , A.S. Turaev 1 , Y.Huang 2 , I.Y. Mexmonov 1
|
v 5,
3, (2019) Poster presentation 170 MULTIFUNCTIONAL POLYSACCHARIDE HYDROGELS FOR WOUND HEALING A.A. Boydedayev 1 , B.I. Muhitdinov 1,2 , D.M. Amonova 1 , A.S. Turaev 1 , Y.Huang 2 , I.Y. Mexmonov 1 , B.A. Sindarov 1 , M.O. Kalonova 1,3 , L.U. Makhmudov 1 , H. Wang 2 1) Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Mirzo Ulugbek St. 83, 100125 Tashkent, Uzbekistan 2) Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Hai Ke Road, 201203 Shanghai, China 3) National University of Uzbekistan, 4 University St. 4, 100174 Tashkent, Uzbekistan The wound healing processes include complex biological and physiological mechanisms such as hemostasis, inflammation, proliferation, and remodeling. Wound healing is frequently considered a serious social problem in burned and diabetic patients especially. However, there are very limited therapeutic materials in use and thus effective wound-healing materials are still have been required. Polysaccharides usually are considered suitable biopolymers for developing wound healing materials with their unique biocompatibility, biodegradability, and high water uptake and retention properties. Among others, hyaluronic acid is a structural and functional biopolymer, largely presents in pericellular coatings and extracellular matrix, an important ingredient of almost all tissue, and plays an important role in wound healing. In this study, hyaluronic acid hydrogels containing the chitosan oligosaccharide and β-glucan polysaccharide were developed. The hydrogel formulation was structured by crosslinking the polysaccharide chains with chitosan oligosaccharides. The hydrogels prepared were structurally analyzed with IR spectroscopy, differential scanning calorimetry (DSC), and SEM observation methods. The wound-healing activities of the samples were studied dorsal wound rat model. In the IR spectra of the hydrogel samples changes in the peaks from amide (I/II/III) were observed at 1310-1558 cm -1 , confirming the crosslinked structure of the samples. The DSC curves of the hydrogel samples differed from that of the initial polysaccharide samples showing an endothermic absorption peak at 40-100°C, and exothermic absorption peaks around 270-350°C due to differing chemical and physical composition of the samples. The SEM observations of lyophilized hydrogels clearly demonstrated that the hydrogels possess irregular macropores and wavy morphological structures. In the wound permeability evaluations executed in the dorsal wound rat model, the β- glucan containing hyaluronic acid-based hydrogel demonstrated a more progressive wound healing effect (wound closure 100%) compared to the control (wound closure 52.3%) or the hydrogel without β-glucan (wound closure 86.7%) at day 14. In conclusion, we through this study developed hyaluronic/chitosan/β-glucan hydrogel for wound treatment applications. The composite hydrogel developed demonstrated promising wound-healing activity in the animal model. The composite hydrogel is expected to be multifunctional with respect to its applicability together with other supporting or therapeutic payloads. The composite hydrogel is under study for evaluation of detailed pharmacological properties. |