Transplantologiya 2014

Transplantologiya - 2014. - № 4. - P. 21-27.
The effects of Peroxiredoxin VI on the preservation of the small intestine in rats after ischemia / reperfusion damage

A.E.Gordeeva¹, M.G.Sharapov¹, V.I.Novoselov¹, E.E.Fesenko¹, A.A.Temnov², M.Sh.Khubutiya²

¹ Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino

² N.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department, Moscow

Contact: Andrew A. Temnov, [e-mail]

Due to its high oxygen requirements, the intestine is extremely susceptible to ischemia/reperfusion (I/R) injury. The aim of this study was to investigate a protective effect of Peroxiredoxin VI (Prx VI) on the small intestine after I/R injury in a rat model of strangulated ileus. An I/R injury in the small intestine strangulation model was induced by the occlusion of the distal ileum loop and mesenterial blood vessels for 60 min followed by a 120-min reperfusion period. In one of 3 experimental groups, the animals received intravenous injections of 10 mg/kg Prx VI at 15 min prior to the intestinal ischemia/reperfusion in the strangulation model. After surgery, specimens of the small intestine tissue were collected for histological examination. The study has shown that the ischemia/reperfusion seriously compromises the integrity of mucosal villi and crypts and is accompanied by a lymphocyte infiltration, an oxidative stress with a serious mucosal loss. A Prx VI administration prior to ischemia/reperfusion protected the rat intestine from the ischemia/reperfusion injury by reducing adverse effects of the oxidative stress and preserving the intestinal mucosa architecture. The study has demonstrated that Prx VI exerts a favourable antioxidant effect and efficiently attenuates ischemia-reperfusion injury of the strangulated intestine segment.