Cost Effective Quantification of Trace Level edta (Ethylene Di-amine Tetra Acetic Acid) by Titrimetry in Active Pharmaceutical Ingredients

Table 1: Calibration data for EDTA
n
EDTA standard (mg)
Cuso4 consumed(ml)
1
0.4792
0.1772
2
0.7188
0.2568
3
0.9584
0.3520
4
1.1980
0.4208
5
1.4376
0.5142
Regression equation
y=0.3497x+0.0090
Correlation coefficient (r
2
)
0.9991
LOD and LOQ (Limit of detection and Limit of quantification) precision
From The linearity study Limit of detection, Limit of quantification was found to be 0.0062% and 0.0189%. But 
the actual response of EDTA By titrimetric LOD,LOQ values are 0.0160% and 0.0350%.We have chosen Limit of 
Detection(LOD) is 0.0160 and Limit of quantification (LOQ) is 0.0350 and it is verified by preparing LOD, LOQ 
precision experiment. The RSD of LOD is less than 33% and LOQ is 10%. The LOD and LOQ Solutions were 
prepared at about predicted concentration levels and analysed six times for checking precision (Table 2).
LOD and LOQ (Limit of detection and Limit of quantification)
The LOD and LOQ Solutions were prepared at predicted concentration and analysed as per the method the RSD was 
found to be less than 10% for LOQ and 33% for LOQ (Table 3).
Precision
System precision
System precision was demonstrated by preparing standard solution at about 0.05% level and performed the titration six times 
and calculated EDTA Content. %RSD (Relative Standard Deviation) less than 5 concerning EDTA Content which indicates 

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Nagaraju et al
Der Chemica Sinica, 2017, 8(1):175-183
Pelagia Research Library
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the acceptable reproducibility and thereby the precision of the system. System precision results are tabulated in (Table 4).